不同剂量地佐辛对丙泊酚逆行性遗忘作用的影响

Impacts of different doses of dezocine on propofol - induced retrograde amnesia

目的观察地佐辛对丙泊酚逆行性遗忘作用的影响。方法按分层随机区组设计将72只昆明种小鼠分成6组（n=12）：生理盐水组（Ns组）、丙泊酚组（P组，200mg·kg^-1）、地佐辛5mg·kg^-1组（D5组）、地佐辛10mg·kg^-1组（D10组）、丙泊酚＋5mg·kg^-1地佐组（PD5组）、丙泊酚＋10mg·kg^-1地佐辛组（PD10组）。通过避暗试验、跳台试验测定各组给药后24、48、72h的潜伏时间和错误次数。结果用药24h后，避暗试验中，与NS组相比，P组潜伏期明缩短（P〈0．05）；与P组相比，地佐辛单用及两药合用组潜伏期均明显延长（P〈0．05或P〈0．01），错误次数均减少（P〈0．05）。跳台试验中，与NS组比较，P组、D10组潜伏期缩短（P〈0．05），PD10组错误次数增多（P〈0．05），但与P组相比，各组潜伏期和错误次数均无统计学差异。48、72h后，各组数据的差异均无统计学意义。结论大剂量腹腔注射丙泊酚具有逆行性遗忘作用，地佐辛可减轻丙泊酚对小鼠学习记忆的影响。

Objective To observe the impacts of different doses of dezocine （D） on the retrograde amnesia induced by propofol （P）. Methods A total of 72 Kunming mice were randomly divided into six groups （n = 12） ： a normal sa- line group （Group NS）, a propofol group （Group P, 200 mg·kg^-1） , a 5 mg·kg^-1 dezocine group （Group D5 ）, a 10 mg·kg^-1dezocine groups （Group Dlo ） , and a propofol and 5 mg·kg^-1 dezocine group （Group PD5 ） and a propofol and 10 mg·kg^-1 dezocine group （Group PD10 ）. The step -down test and step -through test were conducted to recorde the latency period and number of errors of each group 24 h, 48 h, and 72 h after treatment. Results Twenty - four hours later, compared with group NS, the latency time of Group P was obviously shortened in the step - down test （ P 〈 0.05 ）. Compared with Group P, all groups except Group NS showed significantly prolonged latency time （P 〈 0.01 or P 〈 0.05 ）, with less errors （ P 〈 0.05 ）. In the step - through test, compared with Group NS, the latency time of Groups P and D,0 were reduced （ P 〈 0.05 ） , and the number of errors was increased for Group PD10 （ P 〈 0.05 ）. However, compared with Group P, no significant difference was found in latency time and the number of errors. After 48 h and 72 h, no statistical significance was found among these groups. Conclusion A large dose of propofol can cause retrograde amnesia, while its effects on learning and memory in mice can be relieved by administration of dezocine.