miR-143在胰腺癌组织中的表达及临床意义

Clinical significance of expression of miR-143 in pancreatic carcinoma

目的:探究miR-143在胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)组织中的表达与临床参数、预后的相关性.方法:收集70例PDAC患者的癌组织及配对癌旁组织石蜡切片,应用锁定核酸原位杂交(locked nucleic acids-in situ hybridization,LNA-ISH)法检测组织中miR-143的表达水平,收集相关临床资料,并对患者的预后进行随访,采用SPSS16.0进行统计学分析.结果:PDAC癌组织中miR-143阳性表达率明显低于癌旁组织(25.71%vs 68.57%,P=0.001),miR-143的表达与淋巴结转移相关,差异有统计学意义(P=0.045).生存分析结果显示,mi R-143阳性者的预后较好(P=0.026).结论:PDAC患者癌组织中mi R-143呈低表达,且与不良预后相关.

AIM： To investigate the expression of mi R-143in human pancreatic ductal adenocarcinoma（PDAC） and to explore the relation between mi RNA-143 expression and prognosis of patients with PDAC. METHODS： We collected 70 PDAC specimens from patients treated at the First Affiliated Hospital of Zhengzhou University, including cancer tissues and paired cancer-adjacent tissues. The expression of miR-143 was examined by locked nucleic acids-in situ hybridization（LNAISH）. Then the relation between the expression of miR-143 and clinical parameters was analyzed with SPSS16.0. RESULTS： The expression rate of miR-143 was lower in cancerous tissues than in paired non-cancerous pancreatic tissues（25.71% v s 68.57%, P=0.001）. The expression of miR-143 in cancerous tissues was associated with lymph node metastasis（P = 0.045）. The median survival of patients with low mi R-143 expression was significantly shorter than that of patients with high miR-143 expression（P = 0.026）. CONCLUSION： The expression of miR143 is low in cancer tissue, and its low expression is associated with poor prognosis in patients with PDAC.