糖尿病慢性溃疡患者与非糖尿病者脂肪干细胞形态及增殖能力的差异

Difference of morphology and proliferation ability between diabetes mellitus-derived ADSCs and normal ADSCs

目的探讨糖尿病患者与非糖尿病者脂肪干细胞（ADSCs）形态及增殖能力的差异。方法将分别从糖尿病患者与非糖尿病者脂肪组织中分离培养ADSCs，用高倍显微镜（×200）下观察两种细胞镜下形态，用CCK一8法检测两种细胞的增殖能力。结果糖尿病患者ADSCs明显较非糖尿病者的ADSCs肥大，且形状不规则。糖尿病患者ADSCs与非糖尿病者ADSCs的A值第1天分别为0．210±O．002，0．390±O．006；第2天分别为0．250±0．015，0．443±0．023；第3天分别为0．368±0．014，O．726±O．033；第4天分别为0．783±O．060，1.642±O．070；第5天分别为1．767±O．072，3．153±0．067；第6天分别为1．810±0．072，3．170±0．021。第4天起差异有统计学意义（P〈0．05）。结论糖尿病患者ADSCs与非糖尿病者ADSCs在镜下形态上存在明显的差异，并且糖尿病患者ADSCs在增殖能力上也低于非糖尿病者，提示这种差异可能与糖尿病患者溃疡创面难以愈合有紧密的相关性。

Objective To investigate the morphological characteristics and proliferation ability of diabetes mellitus-derived adipose-derived stem cells （ADSCs） by comparing with normal-derived ADSCs. Methods The ADSCs could be achieved from diabetes mellitus＂ adipose tissue by the similar method of normal ADSCs isolation and culture, and then the differnce was compared between the two groups about their morphologies under microscope, and the proliferation ability of two groups was determined by CCK-8. Results ADSCs in patients with diabetes were obvious hypertrophy and irregular in morphology. A values of ADSCs in diabetes and non-diabetes were 0.210±0.002 and 0.390±0.006 in 1st day; 0.250±0.015 and 0.443±0.023 in 2nd day; 0.368±0.014 and 0.726±0.033 in 3rd day; 0.368±0.014 and 0.726±0.033 in 4th day; 1.767±0.072 and 3.153±0.067 in 5th day and 1.810±0.072 and 3.170±0.021 in 6th day, respectively. The difference was statstically significant at the beginning from 4th day （P〈0.05） . Conclusions There is obvious difference of the microscopic morphology between the diabetes mellitus-derived ADSCs and normal-derived ADSCs. The proliferation ability of the diabetes mellitus-derived ADSCs is lower than normal-derived ADSCs. These difference could be closely related to ulcerous non-healing wounds in patients with diabetes mellitus.