银杏叶提取物对新生大鼠缺氧缺血性脑病的保护作用

A Study on Protective Effect of EGB on Hypoxic-Ischemic Encephalopathy in Neonatal Rats.

【目的】 探讨银杏叶提取物 (EGB)对新生大鼠缺氧缺血性脑病 (HIE)的影响。 【方法】 建立HIE新生大鼠模型 ,给予腹腔注射EGB后 ,通过氨基酸分析法、分光光度法以及原位末端标记法对比观察EGB对HIE模型鼠脑组织谷氨酸 (Glu)、天门冬氨酸 (Asp)、一氧化氮 (NO)含量及凋亡细胞百分数的影响。 【结果】 干预组 :Glu( 6 84.3± 5 1.0 ) μg/g ,Asp( 198.4± 34.2 ) μg/g ,NO( 9.5± 4.1) μmol/L ,凋亡细胞百分数 ( 16 .4± 4.2 ) %。对照组 :Glu( 897.5± 82 .6 ) μg/g ;Asp( 2 84.7± 5 3.1) μg/g ;NO( 36 .7± 8.3) μmol/L ;凋亡细胞百分数对照组 ( 35 .7± 4.6 ) %。两组之间差异有非常显著性 (P <0 .0 1)。 【结论】 EGB对HIE有保护作用。

To investigate the protective effect of extract of ginkgo biloba(EGB) on hypoxic-ischemic encephalopathy(HIE) in neonatal rats. The HIE models were made with 7-day-old SD rats,EGB was injected into the HIE models.The contents of glutamate(Glu),aspartate(Asp) and nitric oxide (NO) were measured and the effect on apoptosis of neurons was observed. The values of Glu,Asp and NO and the percentage of apoptosis of neurons in EGB group were lower than those in control group. Glu(684.3±51.0)μg/g,(897.5±82.6)μg/g,ASP(198.4±34.2)μg/g,(284.7±53.1)μg/g;(P<0.01),NO(9.5±4.1)μmol/L,(36.7±8.3)μmol/L(P<0.01).The percentage of apoptosis(16.4±4.2)%,(35.7±4.6)%(P<0.01). [Conclusion] EGB has protective effect on HIE.