摘要
利用AFLP对羽扇豆 (LupinusL .) 3个种 (L .angustifoliusL .,L .albusL .,L .pilosusMurr.)的 18个品种指纹图谱进行了分析 ,4个引物组合共产生 46 2条带、多态性带为 44 2 ,平均每个引物组合产生 110 .5多态性带 ,多态性百分率为 95 .7%。总体平均遗传多样性指数为 0 .781。种间遗传差异大于品种间的遗传差异 ,4个引物组合种间和品种间的平均鉴定效率分别为 10 0 %和 84.7%。按UPGMA方法进行聚类分析 ,所有的供试材料明显地分为 3个组 ,每一个组由同一个种的品种构成。
The genetic difference between species in lupins (Lupinus L.) including L.angustifolius L., L.albus L., L.pilosus Murr. was analyzed using AFLP. 462 bands were developed from 4 primer combinations based on 18 lupins varieties, and among them 442 (95.7%) were polymorphic with an average of 110.5 for per AFLP primer combination. The average genetic diversity index was 0.781 in this study. The genetic difference between species was higher than that of between varieties, the identification percentage of four AFLP primer combinations was 100% and 84.7% between species and between varieties, respectively. The results of cluster analysis indicated that 3 species were distinctly distinguished on the dendrogram using UPGMA method.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第8期911-915,共5页
Scientia Agricultura Sinica
基金
948 9710 12
973 G19980 10 2 0 5项目资助
