芥菜型油菜与甘蓝型油菜种间杂种后代的RAPD分析

RAPD Analysis of the Hybrid Progenies from the Interspecific Cross Brassica juncea × Brassica napus

为了揭示芥菜型油菜与甘蓝型油菜种间杂种后代的遗传分离规律 ,用芥菜型油菜四川黄籽作母本与甘蓝型油菜 10 47自交系杂交 ,杂种F1人工自交或用 10 47自交系花粉回交 ,然后将亲本、杂种F1、F2 和BC1代用 16个随机引物进行PCR扩增。共扩增出 98条带 ,其中双亲共有的带 35条 ,四川黄籽特异带 30条 ,10 47特异带 33条。带型统计分析结果表明在芥甘杂种后代中来自 10 47的遗传物质比来自四川黄籽的遗传物质更能传递给后代 ,这种趋势与杂种后代植株的育性无关 ,在回交后代中四川黄籽的遗传物质比在自交后代中丢失更快。并对造成芥甘种间杂种后代分离扭曲的原因进行了探讨。

This study was carried out in order to uncover the laws of genetic segregation in the interspecific progenies from B. juncea × B. napus. The variety Sichuan Yellow of B. juncea was crossed with the inbred 1047 of B. napus as a male parent. The F 1 hybrid gained was self-pollinated by hand or backcrossed with the inbred 1047 to produce F 2 and BC 1 progenies. The DNAs isolated from the parents, F 1 hybrid, F 2 and BC 1 progenies were amplified for RAPD analysis using 16 10-mer primers. Ninety eight bands were amplified, among which 35 bands were common to both parents, 30 and 33 bands were Sichuan Yellow- and 1047- specific, respectively. Statistical analysis of the band patterns showed that both F 2 and BC 1 progenies from the inbred 1047 inherited more genetic components than from the variety Sichuan Yellow whether the progenies were fertile or not. The genetic component from Sichuan Yellow was eliminated in BC 1 progeny faster than in F 2 progeny. The factors that may cause the distorted segregation in progenies from the interspecific cross B. juncea × B. napus were discussed.