单片式面膜中6种防腐剂的高效液相色谱法测定

Simultaneous determination of six preservatives in facial mask by high performance liquid chromatography

建立了同时测定单片式面膜中苯甲酸、山梨酸、脱氢乙酸、苯氧乙醇、氯苯甘醚和碘丙炔醇丁基氨甲酸酯6种防腐剂的高效液相色谱方法.面膜经50%(体积分数)甲醇提取,采用C18色谱柱,以甲醇和乙酸铵溶液(0.02 mol·L^(- 1))为流动相进行梯度洗脱,流速为1.0 m L·min^(- 1),柱温30℃,苯甲酸、山梨酸、氯苯甘醚、苯氧乙醇和碘丙炔醇丁基氨甲酸酯的检测波长为235 nm,脱氢乙酸的检测波长为295 nm.6种防腐剂浓度在0.5~20μg·m L^(- 1)范围内与峰面积均呈现良好的线性关系,方法的加标回收率为88.1%~105.3%,相对标准偏差为1.7%~2.8%.方法操作简便、快速,重复性好,灵敏度高,可用于面膜中6种防腐剂的测定.

The high performance liquid chromatographic method was developed for the simultaneous determination of six preservatives（ benzoic acid,sorbic acid,dehydro acetic,phenoxyethano,chlorphenesin,iodopropynyl butylcarbamate） in facial mask. The samples were extracted with 50%（ volume fraction） methanol,and then separated on a C18 chromatographic column by gradient elution at a flow rate of 1. 0 m L · min- 1with methanol- ammonium acetate solution as the mobile phase. The column temperature was 30 ℃. The wavelength of detection for benzoic acid,sorbic acid,phenoxyethano,chlorphenesin,iodopropynyl butylcarbamate was 235 nm,but for dehydroacetic acid was295 nm. In the range of 0. 5 to 20 μg·m L- 1the peak area of six preservatives showed a good linear relationship,the recoveries were 88. 1% to 105. 3% with the relative standard deviations 1. 7% to2. 8%. The method is simple,rapid,accurate and sensitive,which can be used for determination of six preservatives in facial mask.