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差示分光光度法测定独一味胶囊中总黄酮含量 被引量:1

Determination of total flavonoids in Kudo capsule by differential spectrophotometry
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摘要 目的:测定藏药独一味胶囊中总黄酮含量。方法:以芦丁为观测指标,在517 nm采用差示分光光度法直接测定,不经分离无干扰。结果:对照品在9.78μg/mL^68.46μg/mL的浓度范围内,差示吸收值(_△A)与浓度呈良好线性关系,r=0.999 7,回归方程为_△A=9.637 5C+0.001 7,平均加样回收率为100.31%,RSD=2.13%(n=6)。结论:该测定方法便于操作,结果准确,重现性良好,可用于藏药独一味胶囊的质量控制。 Objective:To determine total flavonoids in Tibetan Kudo capsule. Methods:Differential spectrophotometry was applied with lutin as standard index at the wavelength of 517 nm without prior separation. Results:The liner range of absorbance and the control concentration was 9.78 μg/mL ~68.46 μg/mL,r =0.999 7. The average recovery was 100.31% and RSD was 2.13%(n=6). Conclusion:The method is simple,accurate,reproducible and suitable for the quality control of Tibetan Kudo capsule.
机构地区 山西中医学院
出处 《山西中医学院学报》 2015年第6期16-17,共2页 Journal of Shanxi College of Traditional Chinese Medicine
基金 山西中医学院"十一五"科技产业规划项目(2006) 山西省科技攻关项目(20120313015-4)
关键词 独一味胶囊 总黄酮 差示分光光度法 Kudo capsule total flavonoids differential spectrophotometry
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