TCF21与Caspase-3在非小细胞肺癌组织中的表达及意义

The expressions of TCF21 and Caspase-3 and their clinical significances in non-small cell lung cancer

目的探讨TCF21与Caspase-3在非小细胞肺癌（NSCLC）组织中的表达及意义。方法收集广西宾阳县人民医院2013-01-2015-01外科手术切除的NSCLC标本共44例（含癌及癌旁组织）。采用实时荧光定量PCR（RT-PCR）技术检测TCF21与Caspase-3在肺癌组织及正常组织中的表达,分析NSCLC组织中TCF21与Caspase-3的表达及与临床分期的关系。结果 TCF21和Caspase-3在NSCLC组织中表达明显低于癌旁正常肺组织[（0.36±0.70）vs（1.00±0.00）、（0.62±0.56）vs（1.00±0.00）,P〈0.01],且二者在肺癌TNMⅢ期中的表达较Ⅰ＋Ⅱ期的表达量明显降低[（0.05±0.06）vs（0.48±0.79）、（0.17±0.20）vs（0.78±0.56）,P〈0.01]。TCF21和Caspase-3在NSCLC组织中的表达呈正相关。结论 TCF21和Caspase-3低表达可能促进了肿瘤的恶性增殖及转移,联合检测TCF21和Caspase-3对判断NSCLC恶性程度、转移潜能及预后有重要的临床意义。

Objective To investigate the relationship between the expressions of TCF21 and Caspase-3 and the clinicopathological features in non-small cell lung cancer（ NSCLC）. Methods 44 samples coming from both adjacent and cancer tissues were collected from the People＇ s Hospital of Binyang County in Guangxi during 2013 to2015. Real-time PCR（ RT-PCR） was used to assay the expressions of TCF21 and Caspase-3 in lung cancer and normal tissues,and the relationship between the expressions of TCF21 and Caspase-3 and the clinicopathological features in NSCLC was analyzed. Results The expressions of TCF21 and Caspase-3 in NSCLC tissues were significantly lower than those in the matched normal lung tissues [（ 0. 36 ± 0. 70） vs（ 1. 00 ± 0. 00）,（ 0. 62 ± 0. 56） vs（ 1. 00 ±0. 00）,P〈 0. 05]. The levels of TCF21 and Caspase-3 expressions were significantly lower in the mid-term lung cancer TNM Ⅲ than those in TNM Ⅰ ＋ Ⅱ[（ 0. 05 ± 0. 06） vs（ 0. 48 ± 0. 79）,（ 0. 17 ± 0. 20） vs（ 0. 78 ± 0. 56）,P〈 0. 01]. There was a positive correlation between the expressions of TCF21 and Caspase-3 in NSCLC. Conclusion The low expressions of TCF21 and Caspase-3 may aggravate the malignant hyperplasy and metastasis of tumor,combined detection of the expressions of TCF21 and Caspase-3 plays an important role in evaluating the degree of malignancy,and predicting the potential metastasis and prognosis of NSCLC.