新疆甜菜坏死黄脉病毒分离物的症状反应及核酸组分分析

Analysis on the symtom reaction of Xinjiang BNYVV isolators and nuclear acid

利用症状反应和RT-PCR技术对12个采自新疆不同地区的甜菜坏死黄脉病毒(BNYVV)分离物进行比较研究,结果表明:12个BNYVV分离物在甜菜、番杏和昆诺阿藜上的症状反应无明显区别,均以黄斑为主;利用RT-PCR进行的核酸组分分析却有一定差异,不同地区的BNYVV分离物含有不同的RNA组分,塔城地区、奇台地区及石河子145团的BNYVV分离物核酸组分为RNA1、RNA2、RNA3和RNA4;库尔勒地区的BNYVV分离物核酸组分还含有RNA5;石河子有些地区的BNYVV分离物核酸组分只有RNA1和RNA2。

The symptom reaction and RT-PCR used to analysis on12Xinjiang BNYVV isolators which collected from different regions of Xinjiang.The results shown that no difference within12BNYVV isolators on sugarbeet,New Zealand ice-plant,fat hen,all have the yellow spot,when using RT-PCR,the composition of nuclear acid have difference,RNA1,RNA2,RNA3and RNA4just found in the isolators of BNYVV from Tatai and145Reg.areas,and RNA5in the Kuerle areas,RNA1,RNA2were found in the Shihezi areas.