化妆品中5种二苯甲酮类紫外吸收剂的液相色谱串联质谱同时测定法

Simultaneous determination of five kinds of benzophenone-type filters in cosmetics by liquid chromatography tandem mass spectrometry

目的建立化妆品中5种二苯甲酮类紫外吸收剂的液相色谱串联质谱同时测定法。方法化妆品样品以甲醇为溶剂溶解,经超声萃取后,以Atlantis C18（2.1 mm×150 mm,5μm）为色谱柱,甲醇（含5%乙腈）-水溶液作为流动相,梯度洗脱,用串联质谱电喷雾负离子模式（ESI-）进行测定,内标法定量。结果 5种二苯甲酮类紫外吸收剂在0.1-50 ng/ml的线性范围内,所得回归方程的线性关系良好,相关系数为0.991 0-0.999 2。方法的检出限为0.001-0.059 ng/ml,定量下限为0.002-0.197 ng/ml,平均回收率为61.9%-115.6%,RSD为2.6%-8.7%。结论该方法简单、准确、灵敏,可用于化妆品中五种二苯甲酮类紫外吸收剂的检测。

Objective To develop a method for the simultaneous determination of five kinds of benzophenone-type filters（BPtype UV filters） in cosmetics by using liquid chromatography tandem mass spectrometry. Methods The cosmetics samples were dissolved in methanol and extracted by the ultrasonic cleaner. The target chemicals were separated on an Atlantis C18column（2.1 mm×150 mm,5 μm） by gradient elution using methanol solution（containing 5% acetonitrile） and water as mobile phases,and determined by liquid chromatography tandem mass spectrometry in ESI under negative ion mode. Results The standard curves for the five BP-type UV filters were linear in the range of 0.1-50 ng/ml,and the correlation coefficients were in the range of 0.991 0-0.999 2,which indicated that the regression equations had a good linearity. The limits of detection were0.005-0.059 ng/ml,and the limits of quantification were from 0.002 ng/ml to 0.197 ng/ml. The average recoveries were 61.8%-115.6% with relative standard deviations of 2.6%-8.7%. Conclusion This method is simple, sensitive and applicable to the determination of five BP-type UV filters in cosmetics.