烟草烟雾对大鼠生精细胞凋亡及血清睾酮和抑制素B水平的影响

Effects of tobacco smoke on apoptosis of rat spermatogenic cells,serum testosterone and inhibin B levels

目的探讨烟草烟雾对大鼠生精细胞凋亡及血清睾酮、抑制素B表达的影响。方法将40只8周龄雄性SD大鼠随机分成4组,分别为对照组及轻、中、重度吸烟组(每天分别暴露于4、8、12支香烟烟雾,早晚各1次),连续染毒8周后成功建立大鼠被动吸烟毒性模型。采用TUNEL法检测睾丸生精细胞凋亡数,分别以直接化学发光和ELISA法检测大鼠血清中睾酮、抑制素B的表达水平。结果对照组及轻、中、重度吸烟组大鼠生精细胞凋亡指数分别为(6.62±0.58)%,(7.08±1.02)%,(8.71±0.76)%,(10.17±1.18)%,各染毒组生精细胞凋亡指数均高于对照组,差异有统计学意义(P<0.01)。各染毒组大鼠血清睾酮、抑制素B水平均低于对照组,且均随染毒剂量的增加而降低,差异有统计学意义(P<0.05)。结论烟草烟雾可诱导大鼠睾丸生精细胞凋亡,并影响血清抑制素B及睾酮分泌。

Objective To investigate the effects of tobacco smoke on the apoptosis of rat spermatogenic cells, serum testosterone and inhibin B levels. Methods Forty 8-week-old male SD mice were randomly divided into four groups, the control group, mild-smoking group, moderate-smoking group and heavy smoking group. After eight weeks of treatment, the mice model with passive smoking toxicity was successfully established. TdT-mediated-dUTP Nick End Labeling （TUNEL） technique were used to detect the apoptosis of spermatogenic cells. The testosterone levels of serum were measured by direct chemiluminescence.The expression of inhibin B in rat serum were measured by enzyme-linked immuno sorbent assay （ELISA）. Results The spermatogenic cells apoptosis index in control group, mild-smoking group,moderate-smoking group and heavy smoking group were （6.62±0.58）%, （7.08±1.02）%, （8.71±0.76）% and （10.17±1.18）% respectively, the number of apoptotic spermatogenic cells in each smoking group were significantly higher than that of control group （P〈0.01）. The levels of inhibin B and testosterone in serum were significantly reduced with the increase of the amount of tobacco smoke compared with the control group （P〈0.05）. Conclusion Tobacco smoke may induce apoptosis of spermatogenic cells and influence the secretion of inhibin B and testosterone.