高产洛伐他汀红曲霉氮离子束诱变育种

Screening of high-yield lovstatin monascus by nitrogen ion beam mutation breeding

为了提高洛伐他汀产量,以红曲霉M14为出发菌株进行N+束诱变。诱变剂量分别为:78×1013、130×1013、182×1013、234×1013N+/cm2,通过高效液相色谱检测诱变菌株发酵产物中洛伐他汀的含量,筛选正突变菌株。结果显示,诱变剂量为130×1013N+/cm2时表现出相对较高的正突变率。最优诱变菌株M50-2洛伐他汀产量4.42 mg/g,相对于出发菌株提高70%。对其进行12次传代培养,发现产洛伐他汀的能力下降了2.3%,表现为良好的遗传稳定性,该菌株具有潜在的应用价值。

In order to improve the yield of lovastatin,strain M14 was injected by ion beam to induce mutant strain. The dose were 78 × 10^13,130 × 10^13,182 × 10^13,and 234 × 10^13N^＋/ cm^2. The positive mutant strains were screened through detection of lovastatin content in the solid-state fermentation products by HPLC. The optimal dose was 130 × 10^13N^＋/ cm^2,which resulted in relatively high positive mutation rate. The best strain M50-2 yield 4. 42 mg / g lovastatin,which was 70% higher than original strains. To examine the genetic stability of M50-2,the strain was continuously sub-cultured to the 12 generation. The ability to produce lovastatin fell by 2. 3%. It remained steady after subculture. This strain has potential application.