伯氏致病杆菌SN52次生代谢产物的分离纯化及结构鉴定

Isolation and Identification of Secondary Metabolites from Xenorhabdus bovienii SN52

采用硅胶柱层析、凝胶LH-20柱层析和半制备高效液相色谱技术对伯氏致病杆菌SN52的次生代谢产物进行分离纯化,得到6个单体化合物。通过波谱综合解析和文献数据对照方法对所分离到的化合物进行结构鉴定。结果显示,均为吲哚类化合物,其中5个为已知化合物,鉴定为:xenocyloins A(1)、xenocyloins B(2)、xenocyloins C(3)、xenocyloins D(4)、xenocyloins E(5);1个为新化合物,命名为xenocyloins F(6)。

Six indole derivatives were isolated and purified from secondary metabolites of Xenorhabdus bovienii SN52 by silica gel column chromatography,Sephadex LH-20 chromatography and semi-preparative high-performance liquid chromatography. Their chemical structures were elucidated by spectroscopic technologies including nuclear magnetic resonance（ NMR） and mass spectroscopy（ MS）,and were identified as xenocyloins A（ 1）,xenocyloins B（ 2）,xenocyloins C（ 3）,xenocyloins D（ 4）,xenocyloins E（ 5） and xenocyloins F（ 6）,in which 6 was a new compound.