系统性硬化症和混合性结缔组织病活动期血清中LOXL-2的比较

Comparison of LOXL-2 Expression between Active Systematic Scleroderma and Active Mixed Connective Tissue Disease

目的比较赖氨酰氧化酶样蛋白2(LOXL-2)在系统性硬化症(SSc)和混合性结缔组织病(MCTD)活动期患者血清中的表达情况。方法用酶联免疫吸附试验(ELISA)法检测20例SSc和20例MCTD患者中LOXL-2的浓度,进行统计分析。结果在活动期MCTD和SSc患者血清中LOXL-2的蛋白表达量高于对照组(均P<0.05),且在SSc患者中LOXL-2浓度与患者皮肤病变评分(mRSS)呈正相关(r=0.982 P=0.001)。结论 LOXL-2在SSc和MCTD活动期患者的血清中高表达,可能与LOXL-2促进组织纤维化及血管炎的发生相关。

Objective To investigate the expression of lysyl oxidase like protein-2 （LOXL-2） in the sera of patients with active systemic scleroder- ma （SSc） and mixed connective tissue disease （MCTD）. Methods An enzyme-linked immunosorbent assay was adopted to measure LOXL-2 in the serum of 20 patients with active SSc, 20 patients with active MCTD, and 20 healthy controls. The measurements among different groups was com- pared, and correlations between LOXL-2 levels and clinical manifestations of SSc and MCTD were examined. Results The levels of LOXL-2 ex- pression in MCTD and SSc groups were significantly higher than those in control group （P 〈 0.05 for all groups）. LOXL-2 expression is also related to the presence of skin lesions in SSc （r = 0.982 P = 0.001 ）. Conclusion High serum level of LOXL-2 in these patients with active SSc and active MCTD suggests that LOXL-2 may be involved in the process of fibrosis and the resulting vasculitis in multiple organs.