脂多糖对人脐静脉内皮细胞Robo4表达的影响

Effect of lipopolysaccharide on expression of Robo4 in human umbilical vein endothelial cells

目的:观察不同浓度脂多糖(LPS)对人脐静脉内皮细胞(HUVECs)Robo4受体表达的作用。方法:体外培养HUVECs,分正常对照组和2个浓度LPS组(正常对照组基础上分别加入终浓度为10μg/m L和100μg/m L LPS),每组重复实验6次。免疫荧光染色及Western blot检测各组细胞Robo4蛋白表达,RT-PCR检测Robo4 m RNA表达。结果:高剂量LPS组HUVECs Robo4蛋白与m RNA表达分别为(0.49±0.08)和(0.23±0.08),较正常对照组(1.35±0.15)和(0.97±0.17)明显下降(P<0.05);低剂量LPS组Robo4蛋白与m RNA表达分别为(0.13±0.13)和(0.94±0.14),较正常对照组均有下降,但差异无统计学意义(P>0.05)。结论:高剂量100μg/m L LPS可下调HUVECs Robo4的表达。

Objective To observe the effects of Lipopolysaccharide（LPS） in different concentrations on expression of Robo4 in human umbilical vein endothelial cells（HUVECs）.Methods HUVECs were cultured in vitro and randomly divided into 3 groups：control group,low dose LPS group（10 μg / m L） and high dose LPS group（100 μg / m L）.Robo4 protein level was detected by immunofluorescence staining and western blot,and Robo4 m RNA level was measured by real-time PCR.Results Robo4 protein and Robo4 m RNA in high dose LPS group were 0.49 ± 0.08 and 0.23 ± 0.08 respectively,which were significantly decreased than those（1.35± 0.15 and 0.97 ± 0.17） in control group（P〈0.05）.However,there were no significant difference between the low dose（1.23 ± 0.13 and 0.94 ± 0.14） group and the control group（P〈0.05）.Conclusion High dose LPS（100 μg / m L） could down-regulate expression of Robo4 in HUVECs.