云斑天牛寄主核桃树皮及树叶的挥发性成分

Volatile components in bark and leaf of host plant Juglans regia L.of Batocera lineolata Chevrolat

【目的】揭示云斑天牛对寄主核桃的选择机制,为筛选对云斑天牛具有生物活性的植物源引诱剂,实现对该虫的生态控制提供理论指导。【方法】采用顶空固相微萃取(Headspace solid phase micro-extraction,HSSPME)结合气相色谱-质谱(Gas chromatograph-mass spectrometry,GC-MS)联用技术,分析了核桃树皮和树叶中的挥发性成分。【结果】核桃树皮和树叶共含有萜烯类、烃类、芳香族化合物、醇类、醛类、酮类、酯类、醌类和杂环化合物9类65种挥发性成分。其中,核桃树皮挥发性成分主要包括9类36种,分别是15种萜烯类、4种烃类、4种芳香族化合物、1种醇类、3种醛类、1种酮类、2种酯类、3种醌类和3种杂环化合物。核桃树叶挥发性成分主要包括8类42种,分别是22种萜烯类、2种烃类、4种芳香族化合物、4种醇类、2种醛类、1种酮类、5种酯类和2种醌类。核桃树皮和树叶均含有的挥发性成分共4类13种,分别是9种萜烯类、1种醛类、1种酯类和2种醌类。此外,核桃树皮和树叶萜烯类相对含量最高,分别为43.371%和78.948%;核桃树皮烃类相对含量最低,为1.043%;核桃树叶酮类和烃类相对含量均较低,分别为0.009%和0.268%。【结论】云斑天牛寄主核桃树皮和树叶挥发性成分主要是萜烯类、烃类、芳香族化合物、醇类、醛类、酮类、酯类、醌类和杂环化合物,其中萜烯类相对含量最高,酮类和烃类相对含量均较低。

【Objective】The volatile components in bark and leaf of host plant Juglans regia L.of Batocera lineolata Chevrolat were determined to explore the selection mechanism of BatoceralineolataChevrolat on host plants and provide references for screening effective attractants of plant origin and ecological control of Batocera lineolata Chevrolat.【Method】Headspace solid phase micro-extraction（HS-SPME）combined with gas chromatograph-mass spectrometry（GC-MS）was selected to determine volatile components in bark and leaf of Juglans regia L.【Result】Bark and leaf of Juglans regia L.contained a total of65 volatile components from 9groups.The bark contained 36 volatile components from 9groups including15 terpenes,4hydrocarbons,4aromatic compounds,1alcohol,3aldehydes,1ketone,2esters,3quinones and 3heterocyclic compounds.The leaf contained 42 volatile components from 8groups including 22 terpenes,2hydrocarbons,4aromatic compounds,4alcohols,2aldehydes,1ketone,5esters and 2quinones.The bark and leaf shared 13 volatile components from 4groups including 9terpenes,1aldehyde,1ester and2 quinones.The relative contents of terpene were the highest in both bark（43.371%）and leaf（78.948%）.Hydrocarbons had the lowest content of 1.043%in bark,while both ketones（0.009%）and hydrocarbons（0.268%）were low in leaf.【Conclusion】The volatile components in bark and leaf of host plant Juglans regia L.of Batocera lineolata Chevrolat included terpenes,hydrocarbons,aromatic compounds,alcohols,aldehydes,ketones,esters,quinones and heterocyclic compounds,among which terpenes had highest content while the contents of ketones and hydrocarbons were lower.