电针胃肠胆腑下合穴对十二指肠溃疡大鼠血清肿瘤坏死因子-α及组织烟碱型乙酰胆碱受体α7等的影响

Effects of Electroacupuncture Stimulation of“Xiajuxu”(ST 39),etc.on Duodenal Mucosal Injury,Serum Pro-inflammatory Factors Levels and Duodenal Nicotinic Acetylcholine Receptor α7 Expression in Duodenal Ulcer Rats

目的:对比观察电针十二指肠溃疡(DU)模型大鼠胃肠胆腑下合穴"下巨虚""足三里""上巨虚""阳陵泉"等穴后对血清及十二指肠组织炎性反应相关物质的影响,探讨小肠下合穴"下巨虚"治疗对应腑病是否存在相对特异性。方法:SD大鼠随机分为空白、模型、下巨虚、足三里、上巨虚、阳陵泉组,每组10只。除空白组外均皮下注射盐酸半胱胺建立DU模型,各穴位组取相应穴位进行电针治疗,每日1次,共治疗10d后,肉眼观察大鼠十二指肠黏膜溃疡情况,ELISA法检测大鼠血清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)及高迁移率族蛋白B 1(HMGB 1)含量,Western blot法检测大鼠十二指肠组织烟碱型乙酰胆碱受体α7(α7nAchR)的表达。结果:与空白组比较,模型组肉眼溃疡评分增高,血清TNF-α、IL-1β及HMGB 1含量均增高(P<0.01),十二指肠中α7nAchR表达水平降低(P<0.01)。与模型组比较,上巨虚、下巨虚、足三里组肉眼溃疡评分依次降低(P<0.05,P<0.01),4个穴位组TNF-α、IL-1β及HMGB 1含量均不同程度降低(P<0.01,除阳陵泉组IL-1β外),而足三里、下巨虚组α7nAchR表达水平增高(P<0.01,P<0.05)。与下巨虚组比较,上巨虚组TNF-α含量增高(P<0.01);阳陵泉组肉眼溃疡评分及TNF-α、IL-1β、HMGB 1含量亦增高(P<0.01,P<0.05),α7nAchR表达水平降低(P<0.05)。与足三里组比较,上巨虚组TNF-α、HMGB 1含量增高(P<0.01,P<0.05);阳陵泉组肉眼溃疡评分及TNF-α、IL-1β、HMGB1含量亦增高(P<0.05,P<0.01),α7nAchR表达水平降低(P<0.05)。结论:1针刺胃肠胆腑下合穴均可通过抑制机体免疫应答,减轻炎性反应,降低黏膜损伤来实现对DU大鼠的干预作用;2下巨虚穴与对应小肠腑之间存在相对特异性联系;3下巨虚、足三里二穴的总体效应基本一致,提示二穴的靶器官效应可能存在部分交集。

Objective To observe the relatively specific effect of electroacupuncture（EA）of＂Xiajuxu＂（ST 39,the lower he-sea point of the small intestine）,etc.on the level of serum TNF-α,Interleukin-1β（IL-1β）and high mobility group protein B 1（HMGB 1）contents,and duodenumα7nicotinic acetylcholine receptor（nAchR）expression in duodenal ulcer rats,so as to explore its mechanisms underlying improving duodenal ulcer.Methods Sixty SD rats were randomly divided into 6groups：normal control,model,Xiajuxu（ST 39）,Zusanli（ST 36）,Shangjuxu（ST 37）and Yanglingquan（GB 34）.The duodenal ulcer model was established by subcutaneous injection of 10% Cysteamine Hydrochloride（300mg/kg）,following by giving the rats with access to water containing Cysteamine.EA（10Hz/50 Hz,1-3mA）was applied to bilateral ST 39,ST 36,ST 37 and GB 34for30 min,once daily for 10 days.The ulcer scores（0-5points）of the duodenal mucosa were assessed according to modified Moraes＇ methods.Serum TNF-α,IL-1βand HMGB 1levels were assayed by ELISA and the expression of neuronalα7nAchR in the duodenal tissue was detected by Western blot.Results After modeling,the ulcer score,serum TNF-α,IL-1βand HMGB 1contents were significantly increased（P0.01）and the expression level ofα7nAchR in the duodenal tissue was significantly downregulated in comparison with the normal control group（P0.01）.Following EA intervention,the serum TNF-αand HMGB 1contents in the Xiajuxu（ST 39）,Zusanli（ST 36）,Shangjuxu（ST 37）and Yanglingquan（GB 34）groups,and the ulcer scores and IL-1βcontent of the Xiajuxu（ST 39）,Zusanli（ST 36）and Shangjuxu（ST 37）groups were considerably reduced,and the expression ofα7nAchR in both Xiajuxu（ST 39）and Zusanli（ST 36）groups was evidently increased（P0.05,P0.01）.No significant changes were found in the ulcer score,serum IL-1βcontent,andα7nAchR expression in the Yanglingquan（GB 34）group andα7nAchR expression in the Shangjuxu（ST 37）group in comparison with the model group（P0.05）.Conclusion EA stimulation of ST 36,ST 37 and ST 39 can reduce ulcer injury in duodenal ulcer model rats,which may be associated with their effects in down-regulating serum TNF-α,IL-1βand HMGB 1contents and up-regulatingα7nAchR expression of the duodenal tissue,possibly by suppressing immune and inflammatory reactions and regulating nicotinic activity.