甘草黄酮抗小鼠肝纤维化药理作用的研究

The Pharmacological Activity of Licoflavone Anti Liver Fibrosis in Mice

目的探讨甘草黄酮抗肝纤维化的药理作用。方法 36只昆明小鼠随机分为三组:A对照组,B模型组,C甘草黄酮预处理组。分别测定血清ALT、AST水平,肝匀浆羟脯氨酸水平,HE染色及Masson三色染色进行肝组织病理学检测。结果经过DMN诱导,模型组小鼠ALT、AST及羟脯氨酸水平明显上升,与模型组相比,甘草黄酮预处理组ALT、AST、羟脯氨酸水平明显下降。肝脏病理学检查结果显示甘草黄酮明显改善了小鼠的肝损伤以及肝纤维化的程度。结论在二甲基亚硝胺诱导的小鼠肝纤维化模型中,甘草黄酮具有明显的抗肝纤维化的药理作用。

Objective To explore the anti-liver fibrosis effect of licoflavone. Methods 36 Kunming mice were divided into three groups randomly：（A） control group（B） fibrosis group（C） Licoflavone pretreatment group. Determine the serum level of ALT and AST, liver homogenate level of hydroxyproline. HE staining and Masson trichromatic staining were used to determine liver tissue. Results The levels of ALT, AST and hydroxyproline were significantly rised by DMN. Compared with fibrosis group, ALT, AST and hydroxyproline levels of licoflavone pretreatment group were significantly reduced. Liver pathology examination results show that licoflavone obviously attenuated the liver injury of mice and the degree of liver fibrosis. Conclusion Licoflavone showed obvious anti-liver fibrosis pharmacological effect in mice induced by DMN.