厄贝沙坦对自发性高血压肾素—血管紧张素—醛固酮系统转化酶2mRNA表达的影响

The Effect of Irbesartan on Angiotensin Converting Enzyme2 mRNA of RAAS Expression in the Spontaneous Hypertensive Patients

目的探讨厄贝沙坦对自发性高血压肾素—血管紧张素—醛固酮系统转换酶2mRNA表达的影响。方法 68例自发性高血压随机分为厄贝沙坦组和对照组,测定各组用药前后患者血压变化,外周血来源巨噬细胞ACE、ACE2 mRNA的含量变化。所获数据采用方差分析和t检验。结果各组治疗4周、8周后血压均降低,厄贝沙坦组降压效果优于对照组(P<0.05),治疗8周后厄贝沙坦组ACE2含量为(20.8±5.1)μl,对照组ACE2含量为(15.9±7.8)u/L,两组比较P<0.05,ACE2mRNA厄贝沙坦组为(0.71±0.07)μl,对照组(0.50±0.03)μl,两组比较P<0.01。结论厄贝沙坦降血压降压效果明显,其降压作用一方面通过与AngⅡAT1受体结合,另一方面通过增加ACE2活性及基因表达实现的。

Objective To observe the effect of irbesartan on the expression of angiotensin converting emzyme 2（ACE2）mRNA of renin-angiotensin-aldosterone system（RAAS） in the spontaneous hyperten-sive patients. Methods68 spontaneous hypertensive patients were randomly divided into two groups： irbesartan group and control group. Then the content of ACE and ACE2 in serum was detected by ELISA, and the expression of ACE mRNA and ACE2 mRNA in spontaneous hypertensive patients was detected by RT-PCR before and after having been treated. The data obtained using analysis of variance and t test. Results After having been treated for 4 weeks and 8 wddks,the blood pressure of patients in two groups were decreased sigifficantly,comparing with control group, irbesartan group seemed to have more obvious therapeutic effect（P〈0.05）, in irbesartan group, the content of ACE2 in serum was increased after having been treated for 8 weeks than that in control group, [（20.8±5.1）u/l VS（15.9±7.8）u/l,P〈0.05 ]; and the expression of ACE2 mRNA in spontaneous hypertensive patients in irbesartan group was obviously increased after 8 weeks comparing with control treatment group [（0.71±0.07）VS（0.50±0.03）, P〈0.01]. Conclusion Irbesartan can reduce blood-pressure by the up-regulation of ACE2 mRNA.