野葛藤地上部分葛根素的提取及稳定性研究

Study on the extraction and stability of pueraria in aerial parts of the Vine of Pueraria lobata

以野葛藤地上部分为研究对象,采用乙醇回流法提取其葛根素,在单因素实验基础上选取影响得率的主要因素:提取温度、提取时间、料液比,通过响应面设计,对野葛藤中的葛根素提取条件进行优化,并研究氧化还原剂和金属离子对野葛藤中的葛根素稳定性的影响。结果表明,野葛藤中的葛根素最佳提取工艺条件为:提取温度为79℃,提取时间为87 min,料液比为1∶14 g/m L;葛根素得率为2.2515%。葛根素的稳定性实验结果表明:氧化剂H_2O_2不影响葛根素稳定性,而还原剂Na_2SO_3导致葛根素吸光度值变化;在金属离子中,Na^+、K^+两种离子不影响葛根素的稳定性,而Cu^(2+)会改变葛根素吸光度值,对葛根素的稳定性有一定影响。因此,葛根素在生产、贮存及使用过程中应注意避免与还原剂和Cu^(2+)离子存在的环境接触。

In this experiment,pueraria was extracted from the Vine of Pueraria lobata using ethanol refluxing method.The main influence factors（ the extraction temperature,the extraction time,the solid- liquid ratio） of extraction rate were selected from the results of single factor test,the extraction conditions of pueraria were optimized by response surface design,and its stability to oxidizer（ reducer） and metal ion was studied.The results showed that the optimum extraction technology was for the extraction temperature 79 ℃,the extraction time was87 min,and the solid- liquid ratio of 1 ∶ 14 g / m L,the extraction rate of pueraria was 2.2515%.The stability results indicated that the pueraria were not affected by H2O2 as the oxidizing agent,but the change of the absorbency was caused by Na2SO3 as the reducing agent.The stability of pueraria was not affected by Na＋and K＋,but it was influenced by Cu^2＋.So the storage and use of the pueraria process should avoid contacting with reducing agent and Cu^2＋.