电针对慢性应激抑郁模型大鼠海马神经血管生成相关蛋白的表达影响

Influence of electroacupuncture on the expression of related proteins in the hippocampus of ratswith chronic stress induced depression

目的观察电针对慢性应激抑郁模型大鼠海马血管内皮生长因子(vascular endothelial growth factor,VEGF)、血管内皮生长因子C(vascular endothelial growth factor-C,VEGF-C)、基质金属蛋白酶8(matrix metalloproteinase-8,MMP-8)、基质金属蛋白酶13(matrix metalloproteinase-13,MMP-13)表达的影响,探讨在慢性应激下电针对抑郁模型大鼠海马神经血管的保护效应。方法将40只雄性SD大鼠随机分为空白组、模型组、电针组、氟西汀组,每组10只。除空白组外,其余组均采用慢性应激结合孤养方法造模28天,电针组和氟西汀组分别于造模前1小时进行电针干预和药物治疗。运用生物素标记蛋白抗体芯片技术,筛选出差异蛋白VEGF、VEGF-C、MMP-8和MMP-13。结果与空白组比较,模型组大鼠海马VEGF、VEGF-C、MMP-8、MMP-13蛋白表达上升(fold change=1.20,1.34,1.25,1.31)。与模型组比较,电针组与氟西汀组:VEGF蛋白表达水平下降(fold change=0.73,0.70);VEGF-C蛋白表达水平下降(fold change=0.66,0.59),MMP-8表达水平下降(fold change=0.70,0.58),MMP-13表达水平下降(fold change=0.74,0.63)。结论电针能够减轻慢性应激对大鼠海马的影响,对神经和血管具有保护作用,这可能是电针抗抑郁作用的分子机制之一。

Objective To observe the effects of electric acupuncture（ EA） on VEGF,VEGF-C,MMP-8 and MMP-13,which are relevant to angiogenesis and injuries in vessels and nerves in hippocampus of CUMS rats. The protective effect of electric acupuncture on the hippocampus neurons of rats with depression induced by chronic stress was explored. Methods 40 SD rats were randomly divided into four groups： blank group,model group,EA group,and fluoxetine group. The chronic unpredictable mild stress model was established. Biotin-labeled protein chip technology were used to detect the expression of VEGF,VEGF-C,MMP-8 and MMP-13. Results Compared with the blank group,the protein expressions of VEGF,VEGF-C,MMP-8 and MMP-13 in the model group were increased（ fold change = 1. 20,1. 34,1. 25,1. 31）. Compared with the model group,VEGF was decreased in EA group and fluoxetine group（ fold change = 0. 73,0. 70）. VEGF-C was decreased in EA group and fluoxetine group（ fold change =0. 66,0. 59）,MMP-8 was decreased in EA group and fluoxetine group（ fold change = 0. 70,0. 58） and MMP-13 was decreased in EA group and fluoxetine group. Conclusion EA can reduce the influence of chronic stress on the hippocampus of rats,and has protective effect on nerve and vessels,which may be one of the molecular mechanisms of anti depression.