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2012—2014年中国八省区5岁以下腹泻儿童中札如病毒感染分析 被引量:9

The analysis of sapovirus infection in children under 5 years old with diarrhea in eight provinces of China, 2012-2014
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摘要 目的了解2012—2014年中国部分地区5岁以下儿童腹泻病例中札如病毒的分子流行病学特征。方法2012年1月-2014年12月收集3175例5岁以下儿童腹泻病例粪便标本和流行病学资料,利用RT.PCR方法检测札如病毒,测定所有阳性毒株的序列并进行系统进化分析。结果札如病毒的总阳性率为0.69%(22/3175),其中6月以下、6—12月、12—24月龄组儿童中札如病毒的阳性率分别为0.53%、0.70%、0.52%,而48—60月龄组的阳性率为2.63%。2012—2014年阳性率分别为1.05%、0.38%和0.50%。系统进化分析表明GI.1型(8株)和GII.1型(7株)札如病毒为主要毒株,其他基因型包括GI.2(2株)、GII.3(1株)、GII.4(1株)、GII.5(2株)、GIV(1株)。结论札如病毒是我国部分地区儿童急性胃肠炎的病原之一,GI.1和GII.1基因型是目前主要的检出型别。 Objective To investigate the molecular epidemiological characteristics of sapovirus in children under 5 years old with diarrhea in eight provinces of China from 2012 to 2014. Me^ods From January 2012 to December 2014, a total of 3175 stool specimens and epidemiologieal data were collected from children under 5 years old with diarrhea. Sapoviruses were detected by one step reverse transcription polymerase chain reaction ( RT-PCR). All sapovirus strains were sequenced and the phylogenetic analyses was performed. Results Sapoviruses were detected in 22 of 3175 specimens (0. 69%). The positive rates of sapoviruses among age groups of 〈6 moths, 6 - 12 months and 12 -24 months were 0. 53%, 0.70% and 0. 52%, respectively. The positive rate was 2. 63% in age group of 4 - 5 years old. Phylogenetic analysis showed that the predominant strains were genotype GI. 1 ( 8 strains) and GII. 1 (7 strains) of sapovirus, and genotype of GI. 2 (2 strains), GII. 3 ( 1 strain), GII. 4 ( 1 strain), GII. 5 (2 strains) and GIV ( 1 strain) were also detected. Conclusions Sapovirus was one of the pathogens causing acute gastroenteritis in some areas of China, and genotype GI. 1 and GII. 1 of sapovirus were the predominant strains.
出处 《国际病毒学杂志》 2016年第2期81-84,共4页 International Journal of Virology
关键词 腹泻儿童 札如病毒 基因型 Diarrhea Children Sapovirus Genotype
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参考文献14

  • 1Liu X, Yamamoto D, Saito M, et al. Molecular detection andcharacterization of sapovirus in hospitalized children with acute gastroenteritis in the Philippines[ J]. J Clin Virol, 2015, 68: 83- 88.
  • 2Choi HL, Suh CI, Park SW, et al. Whole-Genome Sequencing A- nalysis of Sapovirus Detected in South Korea [ J ]. PLoS One, 2015, 10(7): e132328.
  • 3Oka T, Mori K, Iritani N, et al. Human sapovirus classification based on complete capsid nucleotide sequences [ J ]. Arch Virol, 2012, 157(2) : 349-352.
  • 4Oka T, Wang Q, Katayama K, et al. Comprehensive review of hu- man sapoviruses[J]. Clin Microbial Rev, 2015, 28( 1 ) : 32-53.
  • 5Dey SK, Phathammavong O, Ngnyen TD, et al. Seasonal pattern and genotype distribution of sapovirus infection in Japan, 2003- 2009[J]. Epidemiol Infect, 2012, 140(1) : 74-77.
  • 6Johnsen CK, Midgley S, Bottiger B. Genetic diversity of sapovirus infections in Danish children 2005-2007 [J]. J Clin Virol, 2009, 46(3): 265-269.
  • 7Svraka S, Vennema H, van der Veer B, et al. Epidemiology and genotype analysis of emerging sapovirus-associated infections across Europe[J]. J Clin Microbiol, 2010, 48(6) : 2191-2198.
  • 8Jiang X, Huang PW, Zhong WM, et al. Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like calicivir- uses by RT-PCR[J]. J Viral Methods, 1999, 83 (1-2) : 145- 154.
  • 9Wang G, Shen Z, Qian F, et al. Genetic diversity of sapovirus in non-hospitalized adults with sporadic cases of acute gastroenteritis in Shanghai, China[J]. J Clin Virol, 2014, 59(4) : 250-254.
  • 10Malasao R, Maneekarn N, Khamrin P, et al. Genetic diversity of norovirus, sapovirus, and astrovirus isolated from children hospi- talized with acute gastroenteritis in Chiang Mai, Thailand [ J ]. J Med Virol. 2008. 80(10) : 1749-1755.

二级参考文献11

  • 1方肇寅,谢华萍,吕红霞,刘娜,章青,段招军,Duncan Steele,Baoming Jiang,Xi Jiang.1999-2005年我国婴幼儿人杯状病毒腹泻研究[J].病毒学报,2007,23(1):9-15. 被引量:131
  • 2Phan T G., Nguyen T A., Yan H. , et al. Development of a novel protocol for RT-multiplex PCR to detect diarrheal viruses among infants and children with acute gastroenteritis in Eastern Russia[J]. Clin Lab, 2005, 51, 429-35.
  • 3Phan T G, Tuan A N, Nishimura S, Nishimuara T, Yamamoto A, Okitsu S, Ushijima H. Etiologic agents of acute gastroenteritis among Japanese infants and children: virus diversity and genetic analysis of sapovirus [J]. Arch Virol,2005,150 : 1415-1424.
  • 4Malasao R, Maneekarn N, and Khamrin P, et al. Genetic diversity of norovirus, sapovirus, and astrovirus isolated from children hospitalized with acute gastroenteritis in Chiang Mai, Thailand[J]. J Med Virol , 2008 Oct,80(10) :1749-1755.
  • 5Phan T G, Okame M, Nguyen TA, et al. Human astrovirus, norovirus (GI, GII), and sapovirus infections in Pakistani children with diarrhea[J]. J Med Virol, 2004 Jun.73(2) :256-261.
  • 6Dove W, Cunliffe N A, Gondwe J S, Broadhead RL, Molyneux ME, Nakagomi O, Hart CA Detection and characterization of human caliciviruses in hospitalized children with acute gastroenteritis in Blantyre, Malawi [J]. J MedVirol ,2005, 77: 522-527.
  • 7Okada M, Shinozaki K, Ogawa T, Kaiho I. Molecular epidemiology and phylogenetic analysis of Sapporo-like viruses[J]. Arch Virol, 2002) ,147:1445-1451.
  • 8Phan TG, Tuan AN, Nishimura S, Nishimuara T, Yamamoto A, Okitsu S, Ushijima H. Etiologic agents of acute gastroenteritis among Japanese infants and children: virus diversity and genetic analysis of sapovirus [J]. Arch Virol ,2005, 150:1415-1424.
  • 9叶新华,金玉,方肇寅,程卫霞,杨学梅,黄湘,谢华萍.兰州地区2003—2007年急性腹泻住院患儿中札如病毒感染情况研究[J].中华流行病学杂志,2008,29(8):843-844. 被引量:3
  • 10常昭瑞,靳淼,刘娜,谢华萍,崔淑娴,章青,段招军.我国九省区2006年札如病毒流行状况及基因序列分析[J].病毒学报,2009,25(2):113-116. 被引量:25

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