摘要
目的了解2012—2014年中国部分地区5岁以下儿童腹泻病例中札如病毒的分子流行病学特征。方法2012年1月-2014年12月收集3175例5岁以下儿童腹泻病例粪便标本和流行病学资料,利用RT.PCR方法检测札如病毒,测定所有阳性毒株的序列并进行系统进化分析。结果札如病毒的总阳性率为0.69%(22/3175),其中6月以下、6—12月、12—24月龄组儿童中札如病毒的阳性率分别为0.53%、0.70%、0.52%,而48—60月龄组的阳性率为2.63%。2012—2014年阳性率分别为1.05%、0.38%和0.50%。系统进化分析表明GI.1型(8株)和GII.1型(7株)札如病毒为主要毒株,其他基因型包括GI.2(2株)、GII.3(1株)、GII.4(1株)、GII.5(2株)、GIV(1株)。结论札如病毒是我国部分地区儿童急性胃肠炎的病原之一,GI.1和GII.1基因型是目前主要的检出型别。
Objective To investigate the molecular epidemiological characteristics of sapovirus in children under 5 years old with diarrhea in eight provinces of China from 2012 to 2014. Me^ods From January 2012 to December 2014, a total of 3175 stool specimens and epidemiologieal data were collected from children under 5 years old with diarrhea. Sapoviruses were detected by one step reverse transcription polymerase chain reaction ( RT-PCR). All sapovirus strains were sequenced and the phylogenetic analyses was performed. Results Sapoviruses were detected in 22 of 3175 specimens (0. 69%). The positive rates of sapoviruses among age groups of 〈6 moths, 6 - 12 months and 12 -24 months were 0. 53%, 0.70% and 0. 52%, respectively. The positive rate was 2. 63% in age group of 4 - 5 years old. Phylogenetic analysis showed that the predominant strains were genotype GI. 1 ( 8 strains) and GII. 1 (7 strains) of sapovirus, and genotype of GI. 2 (2 strains), GII. 3 ( 1 strain), GII. 4 ( 1 strain), GII. 5 (2 strains) and GIV ( 1 strain) were also detected. Conclusions Sapovirus was one of the pathogens causing acute gastroenteritis in some areas of China, and genotype GI. 1 and GII. 1 of sapovirus were the predominant strains.
出处
《国际病毒学杂志》
2016年第2期81-84,共4页
International Journal of Virology