甲状腺良性结节中VEGF的表达及其与超声造影的关系

Expression of VEGF in Thyroid Benign Nodes and the Relationship with Ultrasound Contrast

目的:研究结节性甲状腺肿和甲状腺腺瘤中血管内皮生长因子(VEGF)的表达情况,并探讨其与超声造影的相关性,为超声诊断提供依据。方法:回顾性分析30例结节性甲状腺肿和30例甲状腺腺瘤患者的超声造影检查结果,根据时间强度曲线获得造影参数。术后标本用Western印迹检测其VEGF表达情况,并与超声造影指标进行相关性分析。结果:30例结节性甲状腺肿患者中24例造影后肿块与周围甲状腺实质等回声,4例出现高回声,2例出现低回声,12例患者有囊性变表现;30例腺瘤28例造影后肿块与周围甲状腺表现为高回声,2例出现等回声,26例肿块内部均匀增强。甲状腺腺瘤组平均渡越时间(m TT)、峰值强度(Imax)大于结节性甲状腺肿组(P<0.05);甲状腺腺瘤较结节性甲状腺肿VEGF表达明显升高(P<0.01)。VEGF表达水平与始增时间(RT)、达峰时间(TTP)、m TT水平呈负相关(P<0.05),与Imax水平呈正相关(P<0.05)。结论:甲状腺结节的超声造影指标与VEGF表达水平存在相关性,可以为结节性甲状腺肿及甲状腺腺瘤的诊治提供依据。

Objective： To investigate the vascular endothelial growth factor（VEGF） levels in nodular goiter andthyroid adenoma, and correlation between them. Methods： 30 cases of nodular goiter and 30 cases of thyroid ade-noma patients were analysed with ultrasound contrast respectively. Imaging parameters were got by time intensitycurves. The VEGF expression in thyroid specimens was analyzed by Western blot, and performed correlation analy-sis with the maging parameters of ultrasound contrast. Results： 26 of the 30 cases with nodular goiter were equalecho, 4 cases were hyper echo and 2 cases were hypo echo. Goiter cystic change was found in 12 cases. 28 ofthe 30 cases with thyroid adenoma were hyper echo, 2 case was equal echo and 26 cases got homogeneous echo.The levels of m TT and Imax in thyroid adenoma were higher than that of nodular goiter（P〈0.05）. We couldfound more VEGF expression in thyroid adenoma than that in nodular goiter（P〈0.01）. The levels of VEGF werenegative correlated with RT, TTP, m TT（P〈0.05）, and positive correlated with Imax. Conclusion： There was an cor-relationship between imaging parameters of ultrasound contrast and VEGF levels, it could provide evidence for thediagnosis and treatment of thyroid benign nodes.