安康地区乙型肝炎病毒基因型分布及临床相关性研究

Distribution and clinical significance of hepatitis B virus genotypes in patients with chronic hepatitis B virusinfection in Ankang

目的:探讨安康地区HBV基因型的分布状况及其与临床的相关性。方法:应用型特异性引物法检测陕西省安康地区1275例HBV感染者的HBV基因型,并分析了287例住院患者的基因型与临床的相关性。结果:1275例HBV感染者中B基因型902例(70.75%),C基因型370例(29.02%),B/C混合型3例(0.23%),未检测到其他基因型。287例住院患者基因型分布在性别上无明显差异(P=0.32),B型、C型患者在携带者、慢性肝炎、重型肝炎、肝硬化和肝癌中的分布无明显差异(P=0.45),在ALT水平及HBV DNA载量上无明显差异(分别为P=0.75,P=0.63);C基因型患者的HBeAg阳性率(70.37%)明显高于B基因型患者(44.17%),P<0.001。结论:安康地区HBV感染者基因型以B型为主,C型次之,C基因型患者的HBeAg阳性率明显高于B基因型患者,B、C基因型在性别、疾病状态、肝脏炎症活动度及ALT水平上无显著性差异。

Objective：To investigate HBV genotypes distribution and relationship between genotypes and clinical significance in Ankang.Methods;By using Polymerase chain reaction- amplification with Sequence-Specific Primers（PCR-SSP） method,we studied the genotypes of HBV of 1275 chronic carriers in Ankang.The relationship between genotypes and clinical significance were analyzed in 287 inpatients.Results;Of the 1275 chronic HBV patients,902（70.75%） were diagnosed with genotype B,370（29.02%） were with genotype C,3（0.23%） were with mixed genotype B and C.The distribution of genotypes was not different between male and female in 1275 patients（P= 0.32）.There was no significance difference among the patients with chronic asymptomatic HBV carriers,chronic hepatitis,liver cirrhosis,liver failure and hepatocellular carcinoma.The level of ALT and HBV DNA were not significantly different among patients with genotype B and C.HBeAg positive rate of genotype C patients was significantly higher than that of genotype B（70.37%vs44.17%,P〈0.001） Conclusion：HBV genotype B was the main genotype prevalent in Ankan area,followed by genotype C.HBeAg positive rate of genotype C is higher than that of genotype B.Patients with genotype B and C have no difference in sex,the severity of liver disease and HBV DNA level.