新型人工纳米红细胞的生物相容性研究

The biocompatibility study of novel artificial Nano- red blood cells

目的对接枝淀粉包裹血红蛋白的新型人造红细胞(GSEhb)进行生物相容性研究。方法通过不同保存条件人工红细胞渗漏试验以及体外实验,包括补体激活以及凝血、溶血实验和体内血小板计数测试实验检测其生物相容性。结果人工红细胞易于保存,对大鼠补体系统C3无明显激活,血浆中凝血酶无增多,凝血因子测定结果合格,溶血率测试合格。输注人工红细胞悬浮液后,对大鼠的血小板无不良影响。结论人工纳米红细胞具有良好的生物相容性,为进一步临床应用提供了基础。

Objective To study the biocompatibility of novel artificial red blood cells substituted by hemoglobin encapsulated with grafted starch. Methods The biocompatibility of artificial red blood cells was assayed respectively by leakage test in different preservation conditions of artificial red blood cells. There were also some other experiments in vitro including complement activation and blood coagulation,hemolytic test and also platelet count in vivo. Results The artificial red blood cells were easy to save and no obvious activation to the complement C3 system. There was no obvious increase in the plasma thrombase,while the detected results of clotting factors and the hemolytic rate were both qualified. There was no adverse effect on rat platelet after infusion of artificial red blood cell suspension.Conclusion The novel artificial red blood cells exhibit good biocompatibility and provide the experimental foundation for further clinical application.