基于石墨相氮化碳的荧光法检测过氧化氢

A novel fluorosensor for rapid,highly sensitive detection of hydrogen peroxide based on graphite carbon nitride nanosheets

利用热解法合成了石墨相氮化碳(g-C_3N_4)纳米片,邻苯二酚(CA)在辣根过氧化物酶(HRP)和H2O2的催化氧化作用下形成的邻苯醌能有效猝灭g-C_3N_4的荧光。研究了不同反应条件对荧光猝灭效率的影响。在最佳条件下,g-C_3N_4的荧光猝灭效果与H_2O_2的浓度之间存在良好的线性关系,其线性范围为5.0×10^(-8)~7.0×10^(-5)mol/L,检出限为1.6×10-8mol/L。方法已用于牛奶中H_2O_2含量的检测。

The highly dispersive, highly fluorescent and biocompatible graphite carbon nitride（ g-C3N4）nanosheets were prepared through a simple pyrolysis method. The catechol（ CA） was employed as the substrate of horseradish peroxidase（ HRP）,and the corresponding oxidation product O-benzoquinone quenched the fluorescence of g-C3N4 effectively. Effects of different experimental conditions were investigated. Under the optimum conditions,the sensor showed a linear response to H2O2 in the range of 5. 0 × 10- 8~ 7. 0 × 10- 5mol / L,and a low detection limit of 1. 6 × 10- 8mol / L. The method was also successfully applied to the determination of H2O2 in raw milk.