顶空固相微萃取-气相色谱/质谱联用分析果汁中愈创木酚和2,6-二溴苯酚

Determination of guaiacol and 2,6-dibromophenol in juice by HS-SPME-GC-MS

建立了HS-SPME-GC-MS分析果汁中脂环酸芽孢杆菌产生的主要代谢产物的方法。采用顶空-固相微萃取捕集目标代谢产物,利用气相色谱-质谱联用对愈创木酚和2,6-二溴苯酚进行定性和定量分析。在优化后的萃取条件下,愈创木酚在0.49~2.0×10~3μg/L和2,6-二溴苯酚在0.42~1.7×10~3μg/L质量浓度范围内线性关系良好,相关系数（r）分别为0.9981和0.9976,回收率为82.1%~125%,相对标准偏差（n=6）为5.2%~6.0%。方法可用于果汁中脂环酸芽孢杆菌主要代谢产物的检测。

To establish a rapid, sensitive headspace SPME-GC-MS to analyze major metabolite for Alicyclobacillus in juice could provide technical support in assessment of Alicyclobacillus pollution. Headspace SPME was used to extract guaiacol and 2,6-dibromophenol for qualitative and quantitative analysis by GC-MS.Under the optimized conditions, this method showed a good linearity in the concentration of 0. 49 ~2. 0 × 103μg / L and 0. 42 ~ 1. 7 × 103μg / L for determination guaiacol and 2,6-dibromophenol,respectively.The correlation coefficients（ r） of guaiacol and 2,6-dibromophenol were 0. 9981 and 0. 9976,respectively. The recoveries of guaiacol and 2,6-dibromophenol were 82. 1% ~ 125% with precision of 5. 2% ~ 6. 0% RSD（ n = 6）. The method is simple,sensitive and rapid,and was suitable for the determination of guaiacol and 2,6-dibromophenol in juice.