大孔树脂纯化短梗五加果花色苷

Purification of Anthocyanins from Acanthopanax sessiliflorus(Rupr.Et Maxim.)Seem Fruit by Macroprous Resin

采用AB-8型大孔树脂纯化短梗五加果花色苷,以吸附率和解吸率为考察指标,确定短梗五加果花色苷的最佳纯化条件:AB-8型大孔树脂对短梗五加果花色苷的静态吸附平衡时间为4 h,静态解吸平衡时间为2 h,上样液pH 2,洗脱液pH 2,洗脱液乙醇体积分数70%,上样流速1 mL/min,质量浓度1 mg/mL,洗脱液的流速1 mL/min。

The anthocyanins from Acanthopanax sessiliflorus（Rupr. Et Maxim.） Seem fruit were purified by using AB-8 macroporous resin, and the optimum purification conditions of anthocyanins from Acanthopanax sessiliflorus（Rupr. Et Maxim.） Seem fruit were obtained by investigating adsorption rate and desorption rate： static adsorption equilibrium time 4 h, static desorption equilibrium time 2 h, anthocyanins pH 2, pH 2 and 70% ethanol as eluent, anthocyanins flow rate 1 mL/min, anthocyanins concentration 1 mg/mL, eluent flow rate 1 mL /min.