菠萝皮中多酚的制备及其清除自由基研究

Study on the Antioxidant Activity of Polyphenols from the Extracts of Pineapple Skin

研究考察了超临界二氧化碳萃取菠萝皮多酚的工艺,采用单因素试验及响应面分析试验研究菠萝皮多酚超临界二氧化碳水萃取菠萝皮中多酚工艺中的超临界二氧化碳萃取压力,超临界二氧化碳萃取温度,超临界二氧化碳萃取时间,超临界二氧化碳萃取液固比值4个因素对多酚得率的影响,并在此基础上进一步响应面分析优化,从而确定最佳工艺条件。结果表明,最优工艺条件为:超临界二氧化碳萃取压力为23 MPa,超临界二氧化碳萃取温度为54℃,超临界二氧化碳萃取时间为1.90 h,超临界二氧化碳萃取液固比值为45 mL/g,在此条件下得到最高的理论得率为60.76 mg/g;采用上述最优提取条件进行菠萝皮多酚的提取试验,实际测得菠萝皮多酚得率为61.12 mg/g,理论预测值与实测值相比无显著差别;菠萝皮多酚清除羟自由基的试验表明其对·OH的抗氧化能力优于VC和柠檬酸。该工艺试验研究验证了超临界二氧化碳萃取方法的高效、节能的优势,这为菠萝皮多酚的产业化提供了有力的理论和工艺基础。

This study investigated the technology of supercritical CO2 extraction of pineapple skin polyphenols. Single factor test and response surface method were used to study the influence of extraction pressure, extraction temperature, extraction time and liquid-solid ratio of supercritical CO2 extraction on the yield of polyphenols in supercritical CO2 extraction process of pineapple skin polyphenol, on the basis of which extraction process was further analyzed and optimized with response surface method, in order to determine the optimum extraction conditions. It was showed that the optimum conditions were： supercritical CO2 extraction pressure of 23 MPa, supercritical CO2 extraction temperature of 54 ℃, supercritical CO2 extraction time of 1.90 h, and supercritical CO2 extraction liquid-solid ratio of 45 mL /g. Under the optimum conditions, the theoretical highest yield of pineapple skin polyphenols was 60.76 mg/g, while the practical yield obtained was 61.12 mg/g. No significant difference was found between theoretical calculation result and experimental result obtained in extraction process. It was indicated by the experiment of hydroxyl radical scavenging that, the antioxidant property of pineapple skin polyphenols on ·OH was better than VC and citric acid. This study verified the efficient and energy-saving advantages of supercritical CO2 extraction method, therefore providing a strong theory and technology foundation for pineapple skin polyphenols industrialization.