摘要
目的：检测ODC和c—myc在不同阶段胃粘膜病变组织中的表达水平，探讨ODC和c—myc的表达与胃癌癌前病变发生的关系和意义。方法：应用逆转录聚合酶链反应和免疫组化方法测定不同阶段的胃粘膜病变组织中ODC和c—mycmRNA及蛋白的表达。结果：ODCmRNA及蛋白在萎缩性胃炎肠化生（CAG与IM）、不典型增生（DYS）及胃癌（GC）组织中的表达水平显著高于浅表性胃炎（CSG）（P〈O．01）。c—mycmRNA及蛋白在胃癌（GC）组织中的表达水平显著高于浅表性胃炎（CSG）、萎缩性胃炎肠化生（CAG withIM）（P〈0．01），ODC和c—myc在中低分化腺癌的表达水平显著高于高分化腺癌（P〈0．05）。ODC与c—myc在胃粘膜癌变多阶段中的表达呈正相关（P〈0．01）。结论：ODC作为致癌因子在胃癌发生中有重要作用，且c—myc通过促进ODC的表达促进细胞增殖，将2指标联合检测，有助于胃癌及其癌前病变的早期诊断。
Objective： To detect the expression of ODC and c - myc in chronic superficial gastritis, intestinal metaplasia, atypical hyperplasia and gastric carcinoma, to explore the correlation and significance of the expression of ODC and c - myc in gastric carcinoma and precancerous lesions. Methods： The expressions of ODC and c -myc were detected by RT -PCR and immunohistochemistry in 18 cases of chronic superficial gastritis（ CSG）, 18 chronic atrophic gastritis with intestinal metaplasia CAG（ with IM）, 12 gastric dysplasia （DYS） and 30 gastric carcinoma （ GC）, to explore the correlation between ODC and c - myc and their relationship with precancerous gastric lesions. Results： The expression of ODC in CAG with IM, DYS and GC was significantly higher than that in CSG（ P 〈0.01 ）. The expression of c - myc was significantly higher in GC comparing with CSG and CAG with IM （ P 〈 0.01 ）. In addition, ODC and c - myc positive immunostaining rates were significantly higher in poorly - differentiated GC than in well - differentiated GC （ P 〈0.01 ）. The expression of ODC was positively correlated with c - myc at different stages of gastric carcinogenesis. Conclusions ： ODC may play an important role as carcinogenic factor, and c - myc promotes cell proliferation by inducing ODC expression. Detecting both markers together may help in early diagnosis of gastric carcinoma.
出处
《中国伤残医学》
2016年第8期12-14,共3页
Chinese Journal of Trauma and Disability Medicine
