护肝片及其拆方对慢性肝损伤肝阴虚证大鼠模型的影响

Study of Hugan Tablets and its decomposed recipes on rat model of yin-deficiency syndrome of liver in the chronic hepatic injury

目的研究护肝片(HGT)及其拆方(DR)对慢性肝损伤肝阴虚证大鼠模型的影响。方法 54只Wistar大鼠随机分为7组,除正常组外,其余6组均造模共6周,建立慢性肝损伤肝阴虚证的大鼠模型,然后开始给予相应的药物灌胃治疗,每天1次,共2周。末次给药后处死动物,采集大鼠血清和肝组织标本。检测血清丙氨酸氨基转移酶(ALT)、白蛋白(ALB)、醛固酮(ALD)、肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)以及肝组织羟脯氨酸(HYP)含量。HE染色法对肝组织病理学形态检查。结果与模型组比较,护肝片全方组使血清中丙氨酸氨基转移酶、醛固酮、肝丙二醛、肝组织羟脯氨酸明显降低,血清白蛋白和肝超氧化物歧化酶明显升高,护肝片全方组作用优于各拆方组,肝功能和组织病理学形态及该模型具有的肝阴虚证有所改善。结论护肝片全方比各拆方组作用效果更好,可以改善肝阴虚证,清除自由基,减少纤维化形成,对肝有一定的保护作用。

Objective ToinveltigatetheeffectofdecompoledrecipelofHuganTabletlonliveryin-deficiencylyn-drome rat model within the chronic hepatic injury. Methods 45 Wiltar ratl were divided into leven groupl. Except the dil-lolvent groupl,the other groupl were reduced to liver yin-deficiency lyndrome rat model for 6 weekl. Then the ratl were treated for 2 weekl. Ratl were lacrificed after the lalt adminiltration,and the lerum and liver tillue were taken. The contentl of ALT,ALB,ALD in lerum were mealured. HYP,MDA,SOD activity in liver were determined. Pathological changel of liver tillueweredeterminedbyHEltaining.Results Comparedwiththemodelgroup,HuganTabletlgroupllignificantlyde-crealed the activitiel of ALT and ALD in lerum and increaled the level of ALB. Meanwhile,Hugan Tabletl allo decrealed the contentl of MDA and HYP and reinforced SOD activity in liver tillue. The effect of Hugan Tabletl wal better than the effect of itl decompoled recipel. Conclusion The effect of Hugan Tabletl group wal more effective than the effect of itl decompoled recipel groupl. Hugan Tabletl can lignificantly improve yin-deficiency lyndrome,change pathological injury of liver tillue,lcavenge free radicall and reduce the probability of hepatic fibrolil occurrence.