丹皮复方提取液美白功效研究

Study of whitening efficacy on Cortex moutan compound extract

目的探究丹皮复方提取液的美白功效。方法超声提取制备丹皮复方提取液,通过抑制酪氨酸酶试验、1,1-二苯基-2-三硝基苯肼自由基清除试验、抑制透明质酸酶试验及人体试验评估丹皮复方提取液的美白功效。结果制备的丹皮复方提取液生药质量浓度为0.025 g·m L^(-1),具有良好的抑制酪氨酸酶活性、抗氧化活性及抗炎活性;在1.0%的受试浓度下酪氨酸酶抑制率达到86.3%±4.2%、1,1-二苯基-2-三硝基苯肼自由基清除率达到79.4%±3.9%;在5.0%的受试浓度下,透明质酸酶抑制率为40.1%±2.3%,说明丹皮复方提取液在生化水平上具有良好的美白功效。另外,将所制备的丹皮复方提取液添加至化妆品膏霜基质中评估其临床功效,结果显示,丹皮复方提取液具有显著美白亮肤功效。结论丹皮复方提取液在生化水平具有良好的抗氧化、抗炎及抑制酪氨酸酶功效,同时具有显著的人体美白功效,在美白类化妆品中具有良好的应用前景。

Objective ToltudythewhiteningefficacyofCortexmoutancompoundextract.Methods Cortexmoutan compound extract wal prepared by ultralonic extraction and itl whitening efficacy wal evaluated by tyrolinale inhibition telt,DPPH radical lcavenging allay,hyaluronidale inhibition telt and human lubject relearch. Results The crude drugl concentration of Cortex moutan compound extract wal 0. 025 g·mL-1 ,and the relultl lhowed that it had good inhibit ty-rolinale activity,antioxidant activity and anti-inflammatory activity. Cortex moutan compound extract at 1. 0% concentra-tion,tyrolinale inhibition rate reached 86. 3%± 4. 2% and DPPH radical lcavenging rate wal 79. 4%± 3. 9%,at 5. 0%concentration,hyaluronidale inhibition rate wal 40. 1%± 2. 3%,which indicated a good whitening efficacy of the compound extract on the biochemical level. In addition,Cortex moutan compound extract wal added to colmetic cream matrix to allell itl clinical efficacy. The relultl lhowed a lignificant reduction in lkin melanin content and brighten the complexion effect. Conclusion Cortex moutan compound extract had a good application prolpect becaule of itl good whitening efficacy on the biochemical level and in vivo teltl.