缺陷假单胞菌HD13发酵过程中生物量和抗菌活性的变化

Biomass and Antibacterial Activity Change of Pseudomonas diminuta HD13

为探索简单快捷检测缺陷假单胞菌发酵过程中生物量和抗真菌病原菌活性的方法,采用OD值法、菌饼法、差量法和活菌计数法对缺陷假单胞菌菌株HD13发酵过程中生物量和抗菌活性的变化进行研究。结果表明:菌株HD13不同浓度的发酵液与其OD值、抑制率呈正相关,在30℃时其最佳发酵时间为4.5d;其发酵液的最佳检测波长为460nm,在此波长下,OD值(x)与活菌数(y1)、发酵物湿重(y2)、发酵物干重(y3)和病原真菌抑制率(y4)的回归方程分别为y1=1.585 6x+0.060 5(R2=0.991 9),y2=7.223 2x+1.080 2(R2=0.991 9),y3=0.951x+0.072 2(R2=0.915 8),y4=84.166x-3.960 1(R2=0.948 2)。运用OD值可以快速、准确和实时地监控菌株HD13发酵过程中生物量和抗菌活性的变化。

To explore the simply detection method of the biomass and antifungal pathogens activity in the fermentation of P.diminuta HD13,the OD value assay,plugs method,delta rule and viable counting method were employed to screen the biomass and antimicrobial activity for plant pathogenic fungus.Results：There was a positive correlation between comparison ferment filtrate and OD value,inhibitory rate;The appropriate fermentation time of HD13 was 4.5dat 30℃ and the best UV wavelength was460 nm,the equations between OD values and the number of viable cells,soaking weight,dry weight,antifungal activity as follows：y1 =1.585 6x ＋0.060 5（R2=0.991 9）,y2 =7.223 2x ＋1.080 2（R2=0.991 9）,y3 =0.951x＋0.072 2（R2=0.915 8）,y4 =84.166x-3.960 1（R2=0.948 2）.The variation of biomass and antimicrobial activity in the fermentation process was rapidity and accurately controlled with OD value assay.