CCR5在炎症性肠病患者肠黏膜的表达及其与β-arrestin2表达的关系

Correlation of CCR5 expression with β-arrestin 2 expression in colonic mucosa of patients with inflammatory bowel disease

目的:通过分析CCR5在炎症性肠病(IBD)患者活检肠黏膜的表达及其与β-arrestin 2表达的相关性,探讨CCR5与β-arrestin 2在IBD发病中的作用。方法:IBD活动期组53例、IBD缓解期组26例和正常对照组30例纳入研究,用En Vision二步免疫组化方法检测活检肠黏膜CCR5和β-arrestin 2的表达。结果:IBD活动期组CCR5阳性表达率及免疫组化评分均高于正常对照组和IBD缓解期组(P<0.05),CCR5表达与IBD活动期组的临床严重程度、病变范围及内镜下分级无明显关联性;β-arrestin 2在IBD活动期组的阳性表达率均明显低于IBD缓解期组和正常对照组(P<0.05),并且在IBD活动期β-arrestin 2表达与CCR5表达呈负相关性(P<0.05)。结论:在IBD活动期组肠黏膜CCR5呈高表达,β-arrestin 2呈明显低表达,CCR5与β-arrestin 2表达呈负相关性。

AIM： To analyze the expression of CCR5 and correlation with the expression of β-arrestin 2 in the intestinal mucosa of the patients with inflammatory bowel disease（ IBD）,so as to study the role of CCR5 and β-arrestin 2in the pathogenesis of IBD. METHODS： Paraffin sections of the colonic mucosa were prepared from 53 patients with active IBD,26 patients with remissive IBD and 30 healthy people. Immunohistochemical En Vision two-step method was used to test the expression of CCR5 and β-arrestin 2 in the biopsic intestinal mucosa. RESULTS： The positive rate,strongly positive rate and immunohistochemical score of CCR5 expression in active IBD were significantly higher than those in normal controls or remissive IBD（ P 0. 05）. No correlation of CCR5 expression with clinical severity,lesion distribution,and endoscopic grade in active IBD was observed. The expression of β-arrestin 2 was significantly lower in active IBD than that in the remissive IBD and normal controls,and there was a negative correlation of β-arrestin 2 expression with CCR5 expression（ P 0. 05）. CONCLUSION： The expression of CCR5 is higher,and expression of β-arrestin 2 is lower,and there is a negative correlation of expression of CCR5 with expression of β-arrestin 2 in intestinal mucosa of the active IBD.