摘要
目的:研究钙库操纵性钙通道(store-operated calcium channels,SOCC)相关功能蛋白ORAI1-3和STIM1-2在人循环纤维细胞(circulating fibrocytes)中的表达及SOCC对人循环纤维细胞分化的影响。方法:采集健康人外周静脉血,分离出单个核细胞,体外培养分化为循环纤维细胞。采用RT-PCR和real-time PCR检测循环纤维细胞中ORAI1-3及STIM1-2的mRNA表达情况,并检测SOCC抑制剂对循环纤维细胞分化的影响。结果:Realtime PCR检测结果显示ORAI1-3和STIM1-2 mRNA在循环纤维细胞中有较高的表达水平,并且SOCC抑制剂SKF-96365对循环纤维细胞分化具有明显的抑制作用。结论:SOCC表达于循环纤维细胞中,并且影响循环纤维细胞的分化。
AIM： To investigate the expression and function of store-operated calcium channels（ SOCC） in human circulating fibrocytes. METHODS： Peripheral blood mononuclear cells（ PBMCs） were isolated and cultured in serum-free media. After 7 d,the PBMCs differentiated into fibrocytes. RT-PCR and real-time PCR were performed to determine the mRNA expression of ORAI1-3 and STIM1-2 in the fibrocytes. SOCC inhibitor SKF-96365 was used to elucidate the role of SOCC in the differentiation of fibrocytes. RESULTS： The results of real-time PCR showed that the mRNA expression of ORAI1-3 and STIM1-2 was positive in cultured fibrocytes. SKF-96365（ 10 μmol / L） significantly inhibited the differentiation of fibrocytes. CONCLUSION： SOCC-related proteins ORAI1-3 and STIM1-2 are abundantly expressed in the fibrocytes,and may play an important role in the differentiation of these cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第4期733-737,744,共6页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81270069)
