TNF-α对根尖乳头干细胞体外增殖及成牙成骨向分化能力的影响

Effects of TNF-α on the Proliferation and Odontoblastic as well as Osteogenic Differentiation of SCAP in Vitro

目的:探讨肿瘤坏死因子-α(TNF-α)对根尖乳头干细胞(SCAP)外增殖及成牙成骨向分化能力的影响。方法:采用酶消化法结合组织块法获得根尖乳头干细胞,免疫荧光免疫组化法鉴定细胞来源,通过不同浓度的TNF-α进行干预后检测对SCAP增殖,矿化成牙成骨能力的影响。结果:MTT检测显示各浓度组TNF-α均能刺激SCAP的体外增殖,10、50mg/L的TNF-α实验组可明显促进SCAP增殖(P<0.05),差异具有统计学意义;茜素红染色显示与对照组(0mg/L TNF-α)相比,实验组(5、10、50mg/L TNF-α)染色明显变浅,矿化结节形成的较小,对照组形成红色结节范围大且呈深橘色;免疫组化染色显示SCAP胞浆中DSP、BSP均有表达,实验组部分细胞胞浆染色且染色较浅,呈褐色为阳性表达,对照组(0 mg/L TNF-α)细胞胞浆完全着色呈深褐色,为强阳性表达。结论:不同浓度TNF-α对SCAP的生长增殖均有促进作用,TNF-α在不同程度上对SCAP矿化及成牙成骨向分化有抑制作用。

Objective：To investigate the effects of TNF-αon the proliferation and odontoblastic and osteogenic differentiation of stem cells from the apical papilla（SCAP）in vitro.Methods：Stem cells were isolated from the apical papillary by combining enzyme digestion with tissue block method,and identified by immunofluorescence and immunocytochemical method.After treated by TNF-α,the effect on the proliferation and mineralized tooth＇s ontogenesis ability were detected in SCAP.Results：TNF-αin each density group can stimulate the proliferation of SCAP in vitro,particularly in 10mg/L and 50mg/L（P〈0.05）.Additionally,in the experimental group,the Alizarin red staining was obvious weaker with smaller mineral node,and the expression of DSP and BSP were downregulated compared to the control group.Conclusion：TNF-αcould promote the proliferation and inhibit the mineralization of SCAP.