吸氧浓度对心肺复苏大鼠S100β蛋白和神经元特异性烯醇化酶表达的影响

Effects of oxygen with different concentrations on the expressions of S100β protein and neuron-specific enolase after cardiopulmonary resuscitation in rats

目的探讨不同给氧浓度对大鼠心肺复苏(CPR)结局的影响。方法选取成年雄性SD大鼠32只,按随机原则分成对照组与复苏组(包括21%氧浓度组、50%氧浓度组及100%氧浓度组),每组8只。对照组仅行麻醉、气管插管和血管穿刺。复苏组建立大鼠心脏骤停(CA)/CPR模型,分别给予21%、50%和100%浓度的氧气。采用ELISA法检测每组复苏成功后0.5、3、6 h大鼠血清S100β蛋白、神经元特异性烯醇化酶(NSE)水平。结果与对照组相比,复苏组大鼠血清S100β蛋白水平均于复苏成功后逐渐升高(P<0.05),并于复苏后3 h达到峰值;复苏组大鼠NSE水平随时间增加逐渐升高(P<0.05)。复苏组组间对比:21%氧浓度组大鼠血清S100β蛋白、NSE水平升高最为明显,100%氧浓度组次之,50%氧浓度组升高程度最低,差异有统计学意义(P<0.05)。结论给予50%浓度氧气较100%、21%浓度氧气更能降低CPR大鼠血清S100β蛋白和NSE水平,并减轻CPR后的继发性脑损伤,从而改善脑功能。

Objective To investigate the effects of oxygen with different concentrations on the outcome of cardiopulmonary resuscitation（ CPR） in rats. Methods Thirty-two SD rats were randomly divided into the control group and resuscitation group,which was subdivided into 21% oxygen concentration group,50% oxygen concentration group and100% oxygen concentration group,with 8 in each group. Anesthesia,tracheal intubation,and vascular puncturation were performed for the control group. Other groups were given 21%,50% and 100% oxygen respectively. ELISA assay was used to detect the levels of of serum S100β protein and neuron-specific enolase（ NES） at 0. 5,3,and 6 h after successful CPR. Results Compared with the control group,the level of serum S100β protein in resuscitation group was higher（ P〈0. 05） and reached the peak at 3h time point,while the level of serum NSE rose stably with the increase of time（ P〈0. 05）. After a successful resuscitation,the risen levels of S100β protein and NSE were as follows： 21%oxygen concentration group〉 100% oxygen concentration group 〉50% oxygen concentration group（ P〈0. 05）.Conclusion Compared with 21% and 100% oxygen,50% oxygen inhalation can decrease the levels of serum S100βprotein and NSE after a successful CPR,minimize secondary brain injury and thus improve the brain functionality.