双酚A对大鼠椎体及股骨骨密度的影响

Effects of bisphenol A on the bone mineral densities of vertebrate and femoral bone in rats

目的观察不同剂量双酚A(BPA)对雌性F344大鼠椎体及股骨骨密度的影响。方法 4周龄雌性F344大鼠,体质量(53.90±4.23)g,随机分为正常对照组、BPA50组[50 mg/(kg·d)]、BPA200组[200 mg/(kg·d)]、BPA400组[400 mg/(kg·d)]和苯甲酸雌二醇(EB)组[1 mg/(kg·3 d)],每组8只。双酚A(溶解于玉米油中)采用灌胃方式给药,EB行腹腔注射给药,正常对照组给予溶剂玉米油等体积灌胃。观察并记录各组精神状态、体毛脱落及体质量变化。药物干预13周后于10%水合氯醛麻醉下,利用双能X线骨密度仪测定椎体及右侧股骨骨密度。结果 BPA200组体质量最低,BPA200、BPA400组体质量与正常对照组比较均降低,差异有统计学意义(P=0.003;P=0.015)。EB组体质量最高,与正常对照组相比差异有统计学意义(P=0.014)。BPA200组椎体骨密度最低,与正常对照组相比差异有统计学意义(P=0.036)。BPA50组右侧股骨骨密度最低,较正常对照组差异有统计学意义(P=0.027)。EB组椎体骨密度较正常对照组明显增加(P=0.018)。结论一定剂量的双酚A可明显抑制大鼠体质量增长并显著降低大鼠椎体及股骨骨密度。

Objective To observe the effects of different doses of bisphenol A（ BPA） on the bone mineral densities（ BMDs） of vertebrate and femoral bone in female F344 rats. Methods A total of 40 female F344 rats aged 4 weeks,bodyweight（ 53. 90 ± 4. 23） g,were randomly divided into 5 groups： control group,BPA50 group[50 mg /（ kg ·d） ],BPA200group[200 mg /（ kg·d） ],BPA400 group[400 mg /（ kg·d） ]and estradiol benzoate（ EB） group[1 mg /（ kg·3 d） ],with 8rats in each group. BPA（ diluted into corn oil） was given via gavage,EB was given via intraperitoneal injection,and the same volume of corn oil was given via gavage to the control group. The changes of mental state,body hair and bodyweight were recorded. The BMDs of vertebrate and femoral bone were measured after 13 weeks＇ exposure by dualenergy X-ray absorptiometry（ DEXA）. Results The bodyweight of the BPA200 group was the lowest（ P = 0. 003）;the bodyweight of EB group was the highest（ P = 0. 014）; the bodyweight of the BPA400 group was significantly lower than that of the control group（ P = 0. 015）. BMD of vertebrate of the BPA200 group was the lowest and lower than thatof the control group（ P = 0. 036）; BMD of femoral bone of the BPA50 group was the lowest and significantly lower than that of the control group（ P = 0. 027）. BMD of vertebrate in the EB group was remarkably higher than that of the control group（ P = 0. 018）. Conclusion A certain doses of BPA can significantly reduce the bodyweight and the BMDs of vertebrate and femoral bone in female F344 rats.