罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ表达的影响

Effects of rosiglitazone and GW9662 on the expression of PPAR-γ in JEG-3 cells

目的探讨罗格列酮与GW9662对人绒癌JEG-3细胞中过氧化物酶体增殖物激活受体-γ(PPAR-γ)表达的影响。方法免疫组化法测定PPAR-γ在正常绒毛组织、葡萄胎组织及绒癌组织中的阳性表达;体外培养JEG-3细胞,将JEG-3细胞分为罗格列酮组、GW9662组和对照组,分别采用Transwell实验检测不同浓度PPAR-γ的激动剂罗格列酮与其抑制剂GW9662对JEG-3细胞侵袭能力的影响,免疫细胞化学、Western blotting及实时荧光定量PCR观察PPAR-γ在细胞中表达水平的变化。结果 PPAR-γ表达于正常绒毛组织、葡萄胎及绒癌组织的细胞核,且PPAR-γ在绒癌组织中的表达明显下调,仅有少数细胞呈弱阳性染色;Transwell实验结果显示,罗格列酮组JEG-3细胞侵袭能力明显减弱,GW9662组细胞侵袭能力增强,两者与对照组比较差异有统计学意义(P<0.05);罗格列酮处理后的JEG-3细胞PPAR-γ表达量下降,GW9662组其表达量升高,且均与对照组比较差异具有统计学意义(P<0.05)。结论罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ的调节是一种负反馈调节,并通过这种调节方式影响绒癌的侵袭能力。

Objective To investigate the effects of peroxisome proliferator-activated receptor gamma（ PPAR-γ） agonist rosiglitazone and antagonist GW9662 on PPAR-γ expression and invasion ability of JEG-3 cells. Methods Immunohistochemistry method was used to detect PPAR-γ expression and distribution in normal chorionic,hydatidiform mole and choriocarcinoma tissues. Then PPAR-γ expression in rosiglitazone,GW9662 and control groups were examined respectively by Transwell assay,immunocytochemistry,Western blotting and q PCR method. Results PPAR-γ was expressed and distributed in cell nucleus of normal chorionic,hydatidiform mole and choriocarcinoma tissues,and its expression was significant down-regulated in choriocarcinoma tissues. Invasion ability of JEG-3 cells treated by rosiglitazone was decreased,while that of GW9662 group was increased. There was a statistical significance between the two groups（ P〈0. 05）. The expression of PPAR-γ in rosiglitazone group was lower than that in GW9662 group（ P〈0. 05）. Conclusion The activity of PPAR-γ is modulated via a negative feedback regulation mechanism by rosiglitazone and GW9662,and can influence the invasion ability of choriocarcinoma cells.