微波干燥对鲜地黄中地黄苷A,D和益母草苷含量的影响

Effect of Microwave Drying Methods on Contents of Rehmaionoside A,Rehmaionoside D and Leonuride in Fresh Rehmanniae Radix

目的：比较不同微波干燥方法对鲜地黄中地黄苷A,D和益母草苷含量的影响,为地黄产地加工与炮制一体化工艺研究提供参考。方法：采用HPLC测定地黄苷A,D和益母草苷含量,Aichrombond-AQ C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-水（5∶95）,流速1 m L·min-1,柱温30℃,检测波长203 nm,进样量10μL。以冷冻干燥法为参考,考察微波干燥对鲜地黄中指标成分含量的影响。结果：地黄苷A以5 k W微波切3~4 mm片时含量为最高（0.249 mg·g-1）,地黄苷D及益母草苷以3 k W微波切1~2 mm片时含量为最高,分别为3.461,6.857 mg·g-1。结论：微波干燥对益母草苷的含量影响最大,对地黄苷D的影响最小;以微波干燥方法来制备鲜地黄饮片具有一定的可行性,对该药材产地加工与炮制一体化有一定参考意义。

Objective： To measure effect exerted by differing microwave drying methods on rehmaionoside A, rehmaionoside D and leonuride in fresh Rehmanniae Radix. Method： Contents of rehmaionoside A,rehmaionoside D and leonuride were determined by HPLC,Aichrombond-AQ C18column（ 4. 6mm × 250 mm,5 μm）,a mobile phase of acetonitrile-water（ 5 ∶ 95） was used,flow rate was 1 m L·min- 1,column temperature was set at 30 ℃,a UV detector was applied with wavelength of 203 nm. Taken freeze drying as a reference,influence of microwave drying on contents of index components in fresh Rehmanniae Radix was investigated. Result： Contents of rehmaionoside A was the highest,which was reached by 5 k W microwave to cut fresh Rehmanniae Radix into slices with thickness of 3-4 mm（ 0. 249 mg ·g- 1）; maximum contents of rehmaionoside D and leonuride were 3. 461, 6. 857 mg ·g- 1,respectively,which were reached by 3 k W microwave to cut fresh Rehmanniae Radix into slices with thickness of 1-2 mm. Conclusion： Microwave drying method has the biggest effect on the content of leonuride and the smallest effect on contents of rehmaionoside D.Adopting microwave drying method in process of preparing fresh Rehmanniae Radix slices is plausible, which provides technology of concocted processing integration of Radix Rehmanniae with some room for thinking.