二冬膏抗NF-κB抑制介导的人肺癌细胞效应探讨

Inhibition Effect of Erdong Gao Mediated by Nuclear Factor-κB Against Human Lung Cancer Cells

目的:探讨二冬膏的抗肿瘤效应,并研究核转录因子κB(NF-κB)在二冬膏抗肿瘤效应中的作用。方法:用四甲基偶氮唑盐比色法(MTT法)检测二冬膏对A549细胞的增殖抑制效应(量效和时效关系);逆转录聚合酶链反应(RT-PCR)法检测二冬膏对A549细胞p65 mRNA表达的影响;采用免疫印迹法(Western blot)检测二冬膏对A549细胞p65蛋白表达的影响。结果:不同剂量组二冬膏含药血清均具有对肺腺癌A549细胞的明显增殖抑制作用(P<0.05),且各自表现出一定时间和剂量相关性,给药干预24 h后细胞增殖均出现抑制,其中以二冬膏中剂量组(3.2 g·kg-1,bid)干预48 h后对细胞增殖的抑制作用最明显。二冬膏中剂量组(3.2 g·kg-1,bid)干预A549细胞24 h后明显抑制p65 mRNA的表达;中剂量组(3.2 g·kg-1,bid)干预A549细胞后48 h对p65蛋白有显著抑制效应。结论:二冬膏可显著抑制人肺腺癌细胞系A549的增殖,通过对NF-κB的抑制介导其抗肿瘤活性。

Objective： To investigate the anti-tumor effect of Erdong Gao and study the effect of nuclear factor-κB（ NF-κB） in Erdong Gao. Method： The dose-and time-related inhibitory effect of Erdong Gao in proliferation of A549 cells was determined by methyl thiazolyl tetrazolium（ MTT） assay. Effect of Erdong Gao on the p65 mRNA expression and protein expression in A549 cells were detected by reverse transcription-PCR（ RTPCR） and Western blot respectively. Result： The MTT test indicated that the proliferation of A549 cells can be significantly inhibited by the drug serum of different concentrations of Erdong Gao（ P 〈 0. 05）,showing certain dose-dependence and time-dependence respectively. The cell proliferation was inhibited after 24 hours of drug intervention. The most obvious inhibitory effect was detected in Erdong Gao middle-dose group（ 3. 2 g·kg- 1,bid）after intervention for 48 hours. The p65 mRNA expression and p65 protein expression in A549 cells were obviously inhibited in Erdong Gao middle-dose group after intervention for 24 hours and 48 hours respectively. Conclusion：Erdong Gao can significantly inhibit the proliferation of A549 cells in human lung adenocarcinoma cells,and its anti-tumor effect might be mediated by inhibiting NF-κB.