超低温保存对玉蝉花种子生理生化特性的影响

Physiological and Biochemical Characteristics of Iris ensata Seeds after Cryopreservation

以玉蝉花种子为试材,将种子自然含水量9.8%分别降至7.2%、5.0%、3.6%、1.9%,液氮保存超24 h后,采用自来水和室温两种方法化冻,测定种子电导率、发芽率、过氧化物酶活性等活力指标,探讨超低温保存对玉蝉花种子生理生化特性的影响及其超低温保存的可行性。结果表明:玉蝉花种子进行超低温保存是可行的,含水量和化冻方式是影响玉蝉花种子超低温保存效果的重要因素,超低温保存可以适度脱水,选择含水量3.6%的种子保存后有较高的发芽率;超低温保存后采用自来水化冻,玉蝉花种子的发芽率、可溶性蛋白质量分数、POD酶活性优于室温化冻,并且细胞膜结构及透性受损伤小。

With Iris ensata seeds materials,we dried their moisture contents from 9.8% to 7.2%,5.0%,3.6% and 1.9%,respectively. After 24-h cryopreservation,we used the tap water thaw and thaw at room temperature to measure the level of various indicators of the vitality of seeds,including germination,conductivity and POD,to explore the influence on physiological and biochemical characteristics of I. ensata seeds after cryopreservation. I. ensata seed cryopreservation is feasible,the moisture and the thaw are important factors affecting cryopreservation effect,the cryopreservation can moderate dehydration,and the moisture content of 3.6% of the seeds have a high germination rate after cryopreservation. After cryopreservation by using tap water to thaw,the germination rate,soluble protein content,and POD activity were better than those thawed at room temperature,and cell membrane structure and permeability were with less damage.