知柏地黄汤对大鼠膜性肾病大剂量激素治疗中的增效减毒作用

Efficacy Enhancing and Toxicity Reducing Effect of Zhibai Dihuang Decoction on Membranous Nephropathy Rats Treating with a Large Dose of Glucocorticoids

探讨了知柏地黄汤对大鼠膜性肾病(Membranous Nephropathy,MN)大剂量激素治疗中的增效减毒作用。针对7周龄SPF级雄性SD大鼠45只,将其分为空白组(C)、模型组(MG)、知柏地黄汤组(ZDD),每组各15只,采用阳离子牛血清白蛋白(C-BSA)、甲泼尼龙琥珀酸钠(MPSS)制备大鼠MN伴激素性骨损伤复合模型,药物干预4周后观察24h尿蛋白、血清白蛋白(ALB)、总蛋白(TP)、甘油三酯(TG)、总胆固醇(TC)、肌酐(Scr)、尿素氮(BUN)及肾脏、股骨头病理及其半定量分析。结果表明:与MG组比较,ZDD组24h尿蛋白、血清TC、TG水平显著降低(P<0.01),血清TP、ALB水平显著升高(P<0.01),肾组织病理损害较轻,免疫复合物IgG、补体C3荧光染色较弱,骨组织病理损害较轻。ZDD在对大鼠MN大剂量激素治疗中,可能具有缓解蛋白尿、调节血脂代谢、改善肾脏病理改变的作用,以及改善激素性骨损伤的作用。

The efficacy enhancing and toxicity reducing effect of Zhibai Dihuang Decoction in treating rats with membranous nephropathy（MN）with a large dose of glucocorticoids were studied,aiming at 45 SPF grade male SD rats aged 7weeks,which were divided into control group（C）,model group（MG）and Zhibai Dihuang Decoction group（ZDD）,with 15 rats in each group.The MN model with steroid induced bone damage of rats was established with the cationic bovine serum albumin（C-BSA）and methylprednisolone sodium succinate（MPSS）.24 h Urinary protein,serum albumin（ALB）,total protein（TP）,triglyceride（TG）,total cholesterol（TC）,creatinine（Scr）,blood urea nitrogen（BUN）,and the pathology of kidney and femoral head and its semiquantitative analysis were observed after the drug intervention for 4weeks.Compared with these of the MG group,in the ZDD group,the levels of 24 hurineprotein,serum TC and TG decreased significantly（P〈0.01）,the levels of serum TP and ALB increased significantly（P〈0.01）,the renal tissue was less damaged in pathology,the fluorescent staining of the immune complexes IgG and complement C3 was weaker,and the bone tissue was less damaged in pathology.Conclusion in the treatment with a large dose of glucocorticoids on rats with MN：ZDD may have the effect of alleviating proteinuria,regulating blood lipid metabolism,improving renal pathological changes and improving the steroid induced bone damage.