离心运动对大鼠股四头肌及腓肠肌中MG53表达的影响

Effect of Eccentric Exercise on Rats MG53 Expression in Quadriceps Femoris and Gastrocnemius Muscle

目的:探讨离心运动对不同时刻雄雌大鼠股四头肌、腓肠肌中MG53蛋白表达变化的影响。方法:将36只SD大鼠(雄雌各半)随机分成安静对照组C,离心运动后即刻组E0和24h组E24,每组12只,雄雌各6只。E组进行19-21m/min,-16°,90min的一次性跑台运动。结果:股四头肌中,与C组雄雌性大鼠相比,相应E0组MG53蛋白表达量具有显著性差异(P<0.01);而E24组的差异不具有统计学意义(P>0.05)。腓肠肌中情况相似,除雌性E24组与C组相比差异具有统计学意义(P<0.05)。性别差异具有统计学意义(P<0.05)的仅在腓肠肌E0组中。结论:MG53可能与剧烈运动导致的肌肉损伤有关,可以考虑作为评价运动性骨骼肌损伤的指标,并且更具灵敏性。

Objective： To investigate the effects of eccentric exercise on muscle MG53 protein expression in rat. Methods： 36 SD rats were randomly divided into three groups,sedentary control group,eccentric exercise group-rats killed immediately and 24 h after eccentric exercise,12 in each group（ 6 male and 6 female）. E exercise on treadmill,with a running speed of 19-21 m / min,- 16°,90 min.Results： In quadriceps femoris,the expression of MG53 protein was increased（ E0 vs C）,with a significantly different（ P 0. 01）. Similar results were obtained from gastrocnemius muscle. There is gender difference in gastrocnemius muscle of E0 group. Conclusions： MG53 may be related to muscle damage caused by strenuous exercise.MG53 can be considered as a maker for evaluating the injury of skeletal muscle.