BRCA2在卵巢癌中的表达及其与临床病理的相关性

Expression of breast cancer suscepbility gene 2 in ovarian carcinoma tissue and its clinicopathological significance

目的比较乳腺癌易感基因2（breast cancer suscepbility gene 2,BRCA2）在卵巢癌组织中的表达及其与临床病理因素的相关性,分析其作为早期卵巢癌诊断基因的可行性。方法采用荧光定量PCR的方法研究BRCA2 RNA在卵巢上皮癌、正常卵巢组织中的表达差异,并进一步应用western-blotting的方法检测BRCA2蛋白的表达。结果其BRCA2 RNA在卵巢癌组织中的表达量低于正常卵巢组织（P〈0.05）;BRCA2 RNA在临床Ⅲ~Ⅳ期的表达量低于临床Ⅰ~Ⅱ期（P〈0.05）。BRCA2 RNA与CA125在卵巢癌中的表达无相关性（P〈0.05）。BRCA2蛋白在卵巢癌中的表达量同样低于正常卵巢组织（P〈0.05）。结论 BRCA2在卵巢癌的发生发展中起着一定作用,其与CA125的联合监测有望提高卵巢癌的阳性诊断率。

Objective To study the expression of breast cancer suscepbility gene 2（BRCA2） in ovarian carcinoma and its clinicopathological significance,to analyse the feasibility of BRCA2 as a diagnostic gene of early ovarian carcinoma. Methods The expression of BRCA2 RNA in ovarian epithelial carcinoma and normal tissues was detected by PCR,then the expression of BRCA2 protein in those tissues was detected by western-blotting.Results The expression level of BRCA2 RNA in ovarian carcinoma was lower than that in normal ovarian tissues（P〈0.05）. The expression level of BRCA2 RNA in stage Ⅲ-Ⅳ was lower than stage Ⅰ-Ⅱ（P〈0.05）. There was no correlation between BRCA2 and CA125 values（P〈0.05）. The expression of BRCA2 protein in ovarian carcinoma was also lower than that in normal ovarian tissues（P〈0.05）. Conclusion BRCA2 might play an important role in ovarian occurrence and development. The simultaneous determination of BRCA2 and CA125 may increase the sensitivity for diagnosis of ovarian carcinoma.