阿里红黄酮对衰老模型小鼠的抗衰老作用

Effects of the Fomes officinalis flavonoids on anti-senile action in the aging model mice

目的:研究阿里红黄酮对衰老模型小鼠的抗衰老作用。方法:将小鼠随机分为6组(n=12):正常对照组,衰老模型组,阳性对照组,低、中、高剂量阿里红黄酮组。除正常对照组外,其余各组均采用D-半乳糖颈背部皮下注射建立亚急性衰老小鼠模型,用不同剂量的阿里红黄酮(100、200和400 mg/(kg·d))灌服小鼠6周后,计算衰老模型小鼠脑指数及脾脏指数、胸腺指数,测定其脑组织丙二醛(MDA)含量及谷胱甘肽过氧化物酶(GSH-Px)活性、肝组织过氧化氢酶(CAT)及超氧化物歧化酶(SOD)活性。结果:阿里红黄酮3个剂量组可不同程度的升高衰老模型小鼠的脑指数、脾脏指数、胸腺指数、脑组织中的GSH-Px活性及肝组织中的CAT、SOD活性,降低其脑组织中的MDA含量。结论:阿里红黄酮可能通过提高机体的抗氧化能力而达到抗衰老作用。

Objective： To study the anti-senile effects of the Fomes offtcinalis flavonoids on the aging model mice. Methods： All Kun ruing mice were randomly divided into six groups, and each group consisted of 12 animals. The groups were categorized as normal, model, positive control groups and low （100 mg/（kg·d））, medium （200 mg/（kg·d））, high （400 mg/（kg·d）） doses of Fomes officinalis flavonoids treated groups. The study was carried out to investigate the cerebral index, spleen index, thymus index, the contents of malondi-aldehyde （MDA） and the activities of glutathione peroxidase （GSH-Px） in brain tissues as well as the activities of catalase （CAT） and superoxide dismutase （SOD） in liver tissues of the aging model mice treated with D-galactose in vivo by subcutaneous injection together with the Fomes officinalis flavonoids at doses of 100, 200 and 400 mg/（kg·d） for 6 weeks. The normal group was administered with 0.20 ml/10 g of normal saline s.c. and 50 ml/kg of normal saline i.g. daily. Results： Compared with the aging model groups, the three doses of the Fomes officinalis flavonoids could elevate the cerebral index, spleen index, thymus index, activities of GSH-Px in brain tissues, the activities of CAT and SOD in liver tissues to different degree and decrease the contents of MDA. Conclusion： The Fomes offtcinalis flavonoids might have anti-senile action by improving antioxidant capacity of human body.