血必净注射液对缺氧/复氧大鼠心肌功能与结构的影响

Effects of xuebijing injection on cardiac function and structure in rats with myocardial hypoxia/reoxygenation injury

目的:探讨不同剂量的血必净注射液对缺氧/复氧大鼠心肌功能的保护作用。方法:采用Langendorff方法制备大鼠离体心脏缺氧/复氧模型。130只雄性SD大鼠随机分为对照组(sham组),缺氧/复氧组(H/R组),低、中、高剂量血必净组(XBJ_L、XBJ_M、XBJ_H组),除对照组外,其他四组按复氧不同时相(复氧0.5 h、1 h、2 h)又分别分为3个亚组(n=10)。对照组在平衡灌注20 min时纪录左室发展压(LVDP)、左心室发展压最大上升/下降速率(±dp/dt_(max))、左心室内压(LVP)、心率(HR)的值,ELISA检测心肌中肌酸激酶同工酶(CK-MB)的浓度,光镜下观察心肌组织结构的改变;其余各组平衡灌注20 min后,灌注ThomasⅡ停搏液使心脏完全停搏30 min之后复灌K-H液使其心脏复跳,连续记录LVDP、±dp/dt_(max)、LVP、HR在复氧不同时间点的动态变化,ELISA检测各组复氧不同时间点心肌中CK-MB的浓度,光镜下观察各组复氧不同时间点心肌组织结构的改变。结果:与sham组相比,其余各组LVDP、±dp/dt_(max)、LVP值均降低(P<0.05),心肌中CK-MB浓度上升(P<0.05),心肌组织结构发生异常改变,随着复氧时间延长,以上指标异常变化逐渐加剧;在复氧0.5 h、1 h、2 h,各剂量血必净组LVDP、±dp/dt_(max)、LVP的值均高于H/R组对应时间点的值(P<0.05),心肌中CK-MB浓度均低于H/R组,心肌组织结构异常变化减轻,以中剂量改善效果最佳(P<0.05)。结论:血必净注射液能够有效改善缺氧/复氧大鼠心肌的功能及形态学结构,以中剂量血必净(4 ml/100 ml)效果最佳。

Objective： To investigate the protective effects of xuebijing （XBJ, Traditional Chinese Medicine Complex） injection on cardiac function in rats with myocardial hypoxia/reoxygenation（H/R） injury. Methods： The isolated langendorff peffused rat heart model was es- tablished. One hundred and thirty SD rats were randomly divided into sham group, hypoxia/reoxygenation group, low dose XBJ（XBJL） group, middle dose XBJ（XBJM） group and high dose XBJ（XBJH） group. All groups except sham group were divided into three subgroups according to reoxygenation time（0.5 h, 1 h, 2 h ） （ n = 10）. In sham group, left ventricular development pressure（LVDP） , maximal rates of increase/decrease of the left ventricular pressure（ ± dp/dtmax ）, left ventricular pressure （LVP）, and heart rates （HR） were recorded after 20 minutes balance perfusion. The creatine kinase-MB （CK-MB） in myocardium was detected by ELISA. In other groups, after 20 minutes balance peffusion, we perfused Thomas Ⅱ to stop the hearts from beating for 30 minutes, then reperfused the K-H until hearts recover beating. The microstructure of myocardium was observed under light microscopy. LVDP, ± dp/dtmax , LVP and HR were continuously recorded in other four groups and the concentrations of CK-MB in myocardium were measured by ELISA at different time points after reoxygenation. Microstrueture of myocardium in each group were observed under light microscopy. Results： LVDP, ± dp/dt max , LVP and HR of other groups were significantly lower than those of sham group（ P 〈 0.05）. The levels of CK-MB were higher than that of sham group（ P 〈 0.05）. LVDP, ± dp/dtmax, LVP and HR of XBJL, XBJM and XBJH groups were higher than those of L/R group at corresponding time points after reoxygenation（ P 〈 0.05）. The levels of CK-MB were lower than that of I/R group（P 〈 0.05） and the cardiac function was improved. The middle dose of XBJ had the best protective effect. Conclusion： Xuebijing injection can effectively improve cardiac function and structure in rats with myocardial hypoxia / reoxygenation injury, and middle dose of XBJ is the best.