干扰miR-21表达抑制胶质瘤U251细胞增殖迁移与侵袭

miR-21 Downregulation Inhibits Cell Proliferation,Migration and Invasion in Glioma

目的探讨干扰miR-21表达对胶质瘤细胞U251增殖、迁移和侵袭的影响。方法使用脂质体将miR-21抑制物(人工合成干扰miR-21表达的核苷酸片段,miR-21处理组)以及对照组转染于U251细胞,利用qRT-PCR验证miR-21处理组转染的细胞中miR-21表达水平;通过MTT法、细胞划痕、transwell等实验观察miR-21处理组和对照组对U251细胞增殖迁移和侵袭的影响。结果 miR-21处理组中miR-21的表达量明显下调。即转染miR-21抑制剂能有效降低U251细胞中miR-21的表达。miR-21处理组的U251细胞与对照细胞相比,增殖速度明显下降,差异具有显著性。细胞划痕实验显示干扰miR-21抑制U251细胞迁移能力。Transwell侵袭实验结果显示,miR-21处理组的U251细胞的侵袭能力较对照组细胞明显下降。结论 miR-21能促进胶质瘤细胞增殖、迁移和侵袭能力,它可能在胶质瘤的发生和进展中发挥重要作用。

Objective This study was designed to investigate the effect of miR-21 knockdown on cell proliferation,migration and invasion of glioma cell line U251. Methods U251 were transfected with miR-21 inhibitor by Lipofectamine.Meanwhile U251 was transfected with NC inhibitor as negative control.qRT-PCR was used to detect the miR-21 expression in these cells.The experiment was divided in negative control inhibitor group and miR-21 inhibitor group.The effects of miR-21 downregulation on cell proliferation,migration and invasion were evaluated by MTT,wound-healing transwell and invasion assays. Results miR-21 expression was remarkably downregulated in miR-21 inhibitor-transfected cells in concentration-dependent manner,indicating transfection with miR-21 inhibitor can effectively reduce expression level of miR-21 in U251 cells.Transfection of miR-21 inhibitor into U251 cells led to a significant decrease in cell proliferation rate compared with control cells（ P〈0.01）.miR-21 inhibitor results in reduction of cell migration（ P〈0.01）.Moreover,the cell invasion by transwell invasion assay was reduced in miR-21-downregulated cells relative to control cells. Conclusions miR-21 can promote cell proliferation,migration and invasion of glioma cells.And it maybe plays an important role in tumorigenesis and development of glioma.