胆宁片对胆汁骨桥蛋白的影响

Effect of Danning tablets on osteopontin in bile

目的探讨中药胆宁片对胆固醇结石形成的预防作用及机制。方法建立骨桥蛋白(OPN)酶联免疫吸附(ELISA)快速检测法,分别检测正常人群、胆固醇以及胆色素结石患者血清及胆汁中的OPN浓度。另取60例术后诊断为胆固醇结石患者按随机序贯原则等分为胆宁片治疗组、熊去氧胆酸治疗组及对照组。观察胆宁片对患者胆汁OPN浓度的影响。结果胆固醇结石患者血清及胆汁中OPN浓度低于正常人群以及胆色素结石患者(P<0.05);胆宁片治疗2周后,胆固醇结石患者血清及胆汁中OPN浓度明显升高(P<0.05)。结论胆宁片能提高血清和胆汁中抑成核因子OPN的浓度,进而降低患者罹患胆固醇结石的风险。

Objective To investigate the mechanism of Danning tablets in the prevention of cholesterol lithogenesis. Methods A rapid method for detection of anti-nucleating factor osteopontin（OPN） was established. OPN concentrations were detected in serum and bile of the normal group, cholesterol-gallstone group and pigmental-gallstone group. Another group of cholesterol-gallstone patients（n = 60） were randomly divided into Danning group, sodium cholate group and control group; and the changes of OPN in serum and bile were observed using ELISA kits. Results The concentrations of OPN in serum and bile in the cholesterol-gallstone patients were significantly higher than those in the pigmental-gallstone and normal groups（P〈 0.05）. After treatment with Danning tablets for 2 weeks, the serum and bile OPN concentrations significantly increased in the cholesterol-gallstone patients（ P〈 0.05）; but this increase was not found in the other two groups.Conclusions Danning tablets can raise the concentrations of anti-nucleating factor OPN in serum and bile,thereby reduce the risk of cholesterol gallstone formation.